The neutrophils from the vaccinated animals were evaluated 30 days after the challenge with M. tuberculosis. The splenocytes were obtained as described above. The cells were incubated for 30 min with APC-conjugated anti-GR1 (BD PharMingen), PercP-conjugated anti-CD14, PE-conjugated anti-CD11c and FITC-conjugated anti-CD11b antibodies (eBioscience). Then, the cells were washed with PBS and fixed with paraformaldehyde using Perm Fix (BD Cytofix). A total of 100,000 events were acquired using BD Biosciences FACSVerseTM and analyzed with the FlowJo 9.0 Software. The neutrophils percentage was determined evaluating singlets that were GR-1+, CD11c-, and CD14-. The total number of neutrophils was obtained multiplying the percentage of neutrophils by the total number of splenocytes from each animal.
Fitc conjugated anti cd11b antibody
Manufactured by Thermo Fisher Scientific
FITC-conjugated anti-CD11b antibodies are laboratory reagents used for the detection and identification of CD11b-expressing cells in flow cytometry and other immunological applications. They provide a specific and sensitive tool for the analysis of CD11b, a cell surface integrin involved in leukocyte adhesion and migration.
Automatically generated - may contain errors
Lab products found in correlation
Apc conjugated anti gr1, by BD (1 mentions)
Pe conjugated anti cd11c, by Thermo Fisher Scientific (1 mentions)
Facsverse, by FlowJo (1 mentions)
Apc cy7 conjugated anti cd11c antibodies, by Thermo Fisher Scientific (1 mentions)
Facscanto 2, by BD (1 mentions)
Fitc conjugated anti cd25 antibody, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti foxp3 antibody, by Thermo Fisher Scientific (1 mentions)
4 protocols using fitc conjugated anti cd11b antibody
1
Neutrophil Evaluation After TB Challenge
2
Quantifying Eosinophils and IL-23R Expression in Lung
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To quantify eosinophils in BAL fluid, cells were stained with FITC-conjugated anti-CD11b antibodies (eBioscience), APC/Cy7-conjugated anti-CD11c antibodies (eBioscience), and PE-conjugated anti-Siglec-F antibodies (BD Biosciences). To measure IL-23 receptor (IL-23R) expression in lung cells, single cells prepared from lung tissue were stained with PerCP-Cy5.5-conjugated anti-CD45 antibodies, FITC-conjugated anti-F4/80 antibodies (eBioscience), APC/Cy7-conjugated anti-CD11c antibodies, APC-conjugated anti-epithelial cell adhesion molecule (EpCAM) antibodies (eBioscience), and PE-conjugated anti-IL-23R antibodies (eBioscience).
3
HL60 Cell Differentiation Induced by RA
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HL60 cells expressing shLacZ or shCASP8 were cultured with or without 1 μM RA for 3 days and then stained with FITC-conjugated anti-CD11b antibody (eBioscience) for 30 min. Flow cytometric analysis was performed with a FACS canto2 (BD Biosciences).
4
Vaccine Effect on Treg and MDSC
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As VEGFR-2 expression is reported on Tregs and MDSCs, the effect of vaccination on normal levels of these cells was assessed as a measure of safety. Splenocytes were isolated when mice were sacrificed and the percentage of Tregs and MDSCs were measured by flow cytometry. Regulatory T cells were stained with a FITC-conjugated anti-CD25 antibody, an APC-conjugated CD4 antibody and a PE-conjugated anti-FoxP3 antibody after fixation and permeabilization with commercial buffers (Ebioscience, Vienna, Austria). MDSCs were stained with a FITC-conjugated anti-CD11b antibody and a PE-conjugated anti-Gr1 antibody (Ebioscience). The cells were analyzed with an Accuri C6 flow cytometer and events in the lymphocyte gate were selected for analysis. Color compensation was based on fluorescence minus one (FMO) controls.
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