Cd8a pe clone53 6.7

Manufactured by Thermo Fisher Scientific

Sourced in United States

CD8a-PE (clone53-6.7) is a fluorescently labeled antibody that binds to the CD8a protein expressed on the surface of certain types of T cells. It is used as a tool in flow cytometry and other immunological applications to identify and quantify CD8a-positive cells.

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CD8a-PE (12 citations)

4 protocols using cd8a pe clone53 6.7

Comprehensive Pharmacological Tools for Research

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Library of Pharmacologically Active Compounds (LOPAC^1280TM),
trovafloxacin, ciprofloxacin, levofloxacin, difloxacin, tosufloxacin,
carbenoxolone, probenecid, Y-27632, blebbistatin, cytochalasin D, purified
nucleotides, suramin and dexamethasone were obtained from Sigma-Aldrich. 7-AAD
and TO-PRO-3 were purchased from Invitrogen. annexin V-FITC, CD8a-PE (clone
53-6.7), CD4-PE-Cy7 (clone RM4-5) and anti-mouse CD16/CD32 (clone 93) were
obtained for eBioscience. Other reagents were obtained as follows: anti-Fas
(clone CH11, Millipore), z-VAD-FMK (Enzo Life Sciences), Q-VD-OPH (SM
Biochemicals) and recombinant apyrase (New England Biolabs).

Poon I.K., Chiu Y.H., Armstrong A.J., Kinchen J.M., Juncadella I.J., Bayliss D.A, & Ravichandran K.S. (2014). Unexpected link between an antibiotic, pannexin channels, and apoptosis. Nature, 507(7492), 329-334.

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Characterization of Tumor-Immune Landscape

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Single cell suspensions were prepared from freshly isolated CT26-HER2 tumors and spleens at sacrifice. The tumor specimens were minced and digested with collagenase (1 mg/mL) for 1 h at 37 °C. The resulting cell suspensions were passed through 70 μm cell strainer and rinsed with flow cytometry buffer (PBS+2% FBS). Spleens were processed directly by means of the cell strainer, and the red blood cells in splenic specimens were lysed by means of ACK buffer (150 mM NH₄Cl, 10 mM NaHCO₃, 1 mM EDTA), samples were pelleted and resuspended in flow cytometry buffer. For each sample, 2 × 10⁶ cells were blocked with α-CD16/32 Ab (clone 93, eBioscience-Thermo Fisher Scientific, Waltham, MA, USA), and then reacted with the antibodies CD4-FITC (clone GK1.5, eBioscience), CD8a-PE (clone 53–6.7, eBioscience), CD45-FITC (clone 30-F11, eBioscience), CD45-Percp-Cy7 (clone 30-F11, eBioscience), FoxP3-PE (clone 150d/e4, eBioscience), CD11b-FITC (clone M1/70, eBioscience), PD-L1-APC (clone MIH5, BD, Franklin Lakes, NJ, USA), and HER2-APC (clone 9G1D4B10, Sinobiological, Beijing, China). The data were acquired by means of BD C6 Accuri. Only the samples that provided at least 1 × 10⁵ events were included in subsequent analysis.

Gianni T., Leoni V., Sanapo M., Parenti F., Bressanin D., Barboni C., Zaghini A., Campadelli-Fiume G, & Vannini A. (2021). Genotype of Immunologically Hot or Cold Tumors Determines the Antitumor Immune Response and Efficacy by Fully Virulent Retargeted oHSV. Viruses, 13(9), 1747.

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Multiparametric Flow Cytometry of Tumor Immune Cells

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Single cell suspensions were prepared from freshly isolated HER2-LLC1 tumors and spleens at sacrifice. Tumors were minced in small pieces and digested with collagenase (1 mg/ml) for 1.5 h at 37°C. The resulting cell suspensions were passed through 70 μm cell strainer and rinsed with flow cytometry buffer. Spleens were processed as described above, and then treated as the tumor samples. Red blood cells in spleen and tumor specimens were lysed by means of ACK buffer, samples were pelleted and resuspended in flow cytometry buffer. Subsequently for each sample 2x10⁶ cells were blocked with α-CD16/32 Ab (clone 93, eBioscience), and then reacted with the antibodies CD4-FITC (clone GK1.5, eBioscience), CD8a-PE (clone 53–6.7, eBioscience), CD45-FITC (clone 30-F11, eBioscience), CD45-Percp-Cy7 (clone 30-F11, eBioscience), CD335-PE (clone 29A1.4, eBioscience), FoxP3-PE (clone 150d/e4, eBioscience), CD11b-FITC (clone M1/70, eBioscience), PD-L1-APC (clone MIH5, BD), CD141-PE (clone LS17-9, eBioscience) and CD69-PercP (clone H1-2F3, eBioscience). Data were acquired on BD C6 Accuri. Only samples which provided at least 100000 events were included in subsequent analysis.

Leoni V., Vannini A., Gatta V., Rambaldi J., Sanapo M., Barboni C., Zaghini A., Nanni P., Lollini P.L., Casiraghi C, & Campadelli-Fiume G. (2018). A fully-virulent retargeted oncolytic HSV armed with IL-12 elicits local immunity and vaccine therapy towards distant tumors. PLoS Pathogens, 14(8), e1007209.

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