Carbopol–934, hydrazine hydrate, 1,1-diphenyl-2-picrylhydrazyl free radicals (DPPH), Dulbeccos Modified Eagle Media (DMEM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), fetal bovine serum (FBS), penicillin, streptomycin—Sigma Aldrich, (St. Louis, MO, USA). Ethanol, glycerol, sulphuric acid (H2SO4), ortho–phosphoric acid 85% (H3PO4), Hydrogen Peroxide 30% (H2O2)—Merck Millipore, (Mumbai, India). Potassium permanganate (KMnO4), hydrochloric acid (HCl), glycerol, methyl paraben, D–Sorbitol—SRL Chemical, (Mumbai, India).
Ortho phosphoric acid 85
Manufactured by Merck Group
Sourced in Germany
Ortho-phosphoric acid 85% is a highly concentrated form of phosphoric acid. It is a clear, colorless, and odorless liquid that is widely used in various industrial and laboratory applications. The primary function of this product is to provide a source of phosphoric acid for chemical reactions, pH adjustments, and other processes that require a strong acid.
Automatically generated - may contain errors
Lab products found in correlation
Potassium dihydrogen phosphate, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Sulphuric acid, by Merck Group (1 mentions)
Hydrazine hydrate, by Merck Group (1 mentions)
Carbopol 934, by Merck Group (1 mentions)
Hydrogen peroxide 30, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
L cysteine, by Merck Group (1 mentions)
Gallic acid g7384, by Merck Group (1 mentions)
2695 separations module, by Waters Corporation (1 mentions)
Waters alliance hplc system, by Waters Corporation (1 mentions)
Disodium hydrogen phosphate anhydrous, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Sodium phosphate monobasic monohydrate, by Merck Group (1 mentions)
2 2 azino bis, by Merck Group (1 mentions)
7 protocols using ortho phosphoric acid 85
1
Antioxidant Evaluation of Carbopol-934 Hydrogels
2
Aniline Oxidation by Alcohol Oxidase
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Aniline (AR-grade), alcohol oxidase (AOX) (A2404, EC1.1.3.13, 10–40 units/mg protein, from Pichia pastoris), ascorbic acid (A5960), gallic acid (G7384) and l-cysteine (W326305) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Absolute ethanol (>99.5%), methanol, orthophosphoric acid 85% and sodium hydroxide (pellets) were delivered by Merck (Nottingham, UK). All chemicals were of analytical reagent grade. The Milli-Q water used was obtained from a Millipore Direct-QTM 5 purification system. Stock solutions of ethanol was prepared daily in 0.1 M phosphate buffer of suitable pH and stored at 4 °C in refrigerator. The phosphate buffer 0.1 M solutions of pH values between 4 and 8 were used for pH studies. The pH was measured using a commercial glass electrode and a pH-meter (model 9318, Hanna Instruments, Woonsocket, RL, USA) calibrated at the pH values of 4.00, 7.00 and 9.00.
3
Silk Fibroin Scaffolds Cryostructuring and Mechanical Testing
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Silk fibroin solutions with different concentrations were pipetted in petri
dishes. Regarding the cryostructuring process and the compressive strength
testing, samples were prepared in petri dishes with a diameter of 35 mm and a
height of 10 mm (V = 6 mL). The tensile strength was
investigated using specimens with bigger dimensions which were produced in petri
dishes with a diameter of 94 mm and a height of 16 mm
(V = 30 mL). The samples were placed horizontally in a freezer
at −20°C. The frozen small samples (ø = 35 mm) were incubated in 100 mL of a
2.5 wt% or 5 wt% solution of anhydrous monocalcium phosphate (MCPA; Budenheim,
Budenheim, Germany) acidified with 2 mL of ortho-phosphoric acid 85% (Merck).
The bigger silk fibroin scaffolds (ø = 94 mm) were immersed in 200 mL of
MCPA-phosphoric acid solution. All samples were stored for 3 or 6 days in the
phosphate solution.
dishes. Regarding the cryostructuring process and the compressive strength
testing, samples were prepared in petri dishes with a diameter of 35 mm and a
height of 10 mm (V = 6 mL). The tensile strength was
investigated using specimens with bigger dimensions which were produced in petri
dishes with a diameter of 94 mm and a height of 16 mm
(V = 30 mL). The samples were placed horizontally in a freezer
at −20°C. The frozen small samples (ø = 35 mm) were incubated in 100 mL of a
2.5 wt% or 5 wt% solution of anhydrous monocalcium phosphate (MCPA; Budenheim,
Budenheim, Germany) acidified with 2 mL of ortho-phosphoric acid 85% (Merck).
The bigger silk fibroin scaffolds (ø = 94 mm) were immersed in 200 mL of
MCPA-phosphoric acid solution. All samples were stored for 3 or 6 days in the
phosphate solution.
4
Formulation and Analysis of Racemic Alpha Lipoic Acid Tablets
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API grade alpha lipoic acid active substance was obtained from Olon SPA Company (Milano-Italy) in racemic form. Lowsubstituted hydroxypropyl cellulose (Shin-Etsu Chemical Co.
-Japan), hydroxypropyl cellulose (Nippon Soda Co. -Japan), magnesium stearate (FACI SPA -Spain), and hypromellose based coating materials (Colorcon, Inc. -England) were used as inactive ingredients in formulations. Analytical grades of potassium dihydrogen phosphate (Merck, Germany), phosphoric acid (ortho-phosphoric acid 85%, Merck, Germany), methanol (J.T. Baker, Poland) and acetonitrile (J.T. Baker, Poland) were used in HPLC analysis. Quantitative stability indicating HPLC test methods were performed on Waters Alliance HPLC System equipped with the 2695 Separations Module (Waters, Milford, MA, USA) with variable wavelength UV-Detector and run with Empower-2 Software. Ultrapure deionized water was obtained from a Millipore water purification system (Millipore Corp., Bedford, MA, USA).
In all studies, the test product (T) alpha lipoic acid 600 mg HR film coated tablet manufactured by ILKO Pharmaceuticals, Turkey (Lot: 1305119001) and the reference product (R) Thioc-tacid® (racemic alpha lipoic acid) 600 mg HR film coated tablet manufactured by Meda Pharma, Germany (Lot: 3741051) were used (Table 1).
-Japan), hydroxypropyl cellulose (Nippon Soda Co. -Japan), magnesium stearate (FACI SPA -Spain), and hypromellose based coating materials (Colorcon, Inc. -England) were used as inactive ingredients in formulations. Analytical grades of potassium dihydrogen phosphate (Merck, Germany), phosphoric acid (ortho-phosphoric acid 85%, Merck, Germany), methanol (J.T. Baker, Poland) and acetonitrile (J.T. Baker, Poland) were used in HPLC analysis. Quantitative stability indicating HPLC test methods were performed on Waters Alliance HPLC System equipped with the 2695 Separations Module (Waters, Milford, MA, USA) with variable wavelength UV-Detector and run with Empower-2 Software. Ultrapure deionized water was obtained from a Millipore water purification system (Millipore Corp., Bedford, MA, USA).
In all studies, the test product (T) alpha lipoic acid 600 mg HR film coated tablet manufactured by ILKO Pharmaceuticals, Turkey (Lot: 1305119001) and the reference product (R) Thioc-tacid® (racemic alpha lipoic acid) 600 mg HR film coated tablet manufactured by Meda Pharma, Germany (Lot: 3741051) were used (Table 1).
5
Micronized Budesonide Particles ALD Synthesis
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Micronized
budesonide particles with a particle
size distribution ranging from 0.1 to 10 μm and a specific surface
area of ∼5.6 m2/g were provided by AstraZeneca and
used as received.32 (link) The ALD precursors,
trimethylaluminum (TMA), titanium tetrachloride (TiCl4),
and silicon tetrachloride (SiCl4), were purchased from
Nouryon, Strem Chemicals, and Alfa Aesar, respectively, and used as
received. Ozone was employed as a co-reactant with TMA, whereas demineralized
water as a co-reactant with TiCl4 and SiCl4.
Each precursor was stored in a stainless steel bubbler under an inert
atmosphere. Sodium phosphate monobasic monohydrate, disodium hydrogen
phosphate anhydrous, sodium dodecyl sulfate, orthophosphoric acid
(85%), and potassium dihydrogen phosphate were purchased from Sigma-Aldrich
and used to prepare sodium phosphate buffer solutions at pH = 6.8.
European Pharmacopoeia reference standard budesonide, supplied by
Sigma-Aldrich, was used for calibration in the in vitro dissolution test. Acetonitrile and ethanol of HPLC grade were provided
by Carl Roth GmbH.
budesonide particles with a particle
size distribution ranging from 0.1 to 10 μm and a specific surface
area of ∼5.6 m2/g were provided by AstraZeneca and
used as received.32 (link) The ALD precursors,
trimethylaluminum (TMA), titanium tetrachloride (TiCl4),
and silicon tetrachloride (SiCl4), were purchased from
Nouryon, Strem Chemicals, and Alfa Aesar, respectively, and used as
received. Ozone was employed as a co-reactant with TMA, whereas demineralized
water as a co-reactant with TiCl4 and SiCl4.
Each precursor was stored in a stainless steel bubbler under an inert
atmosphere. Sodium phosphate monobasic monohydrate, disodium hydrogen
phosphate anhydrous, sodium dodecyl sulfate, orthophosphoric acid
(85%), and potassium dihydrogen phosphate were purchased from Sigma-Aldrich
and used to prepare sodium phosphate buffer solutions at pH = 6.8.
European Pharmacopoeia reference standard budesonide, supplied by
Sigma-Aldrich, was used for calibration in the in vitro dissolution test. Acetonitrile and ethanol of HPLC grade were provided
by Carl Roth GmbH.
6
Laccase from Aspergillus for Indigo Decolorization
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Laccase from Aspergillus spp. (LAsp) with a nominal activity of 1000 U.g−1 of protein, 2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonic acid) (ABTS, ≥98%), Bovine serum albumin (BSA), ethyl alcohol anhydrous, orthophosphoric acid 85%, and Coomassie Brilliant Blue G-250 dye were purchased from Sigma-Aldrich (Rio de Janeiro, Brazil). LAsp is a similar product to Novozym 51003, which is a formulation used for indigo dye decolorization in denim finishing operations and includes a buffer and an enzyme mediator, but this information was not indicated by Novozymes [54 (link)]. Lignocellulosic waste from corn cob (CC), which was used as a support, were collected in supply and processing centers in the city of Imperatriz, State of Maranhão (Brazil). All reagents used were of analytical grade.
7
Monoclonal Antibody Biotinylation Protocol
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The chemicals used in the present study included: NaCO3 and NaCl (El Nasr pharmaceutical chemicals Co., Egypt), NaHCO3 (Biowest Co., USA), Tris/Trizma base and Tween 80 (Sigma, USA), Orthophosphoric acid 85% (Sigma-Aldrich, GmbH, Germany) and Acetic Acid, glacial extra pure (Sham lab, Syria). The reagents used in the present study included: BupH carbonate–bicarbonate buffer, 0.01 M Tris-Buffered Saline (TBS), TBST (TBS containing 0.05% Tween 80). Therapeutic monoclonal antibody trastuzumab (Tmab) was supplied by Roche Diagnostics (GmbH, Germany), Streptavidin poly -HRP was obtained from Pierce (Pierce Biotechnology, Rockford, IL, N200) and Biotin-Trastuzumab Conjugate (B-Tmab) was prepared by labeling of therapeutic humanized monoclonal antibody Trastuzumab (Tmab) using EZ-Link Sulfo-NHS-LC-Biotinylation Kits (Pierce, 21,435) according to manufacturer’s instructions.
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