Polyethylene glycol (PEG) 4000 Da (A16151) was purchased from Alpha-Aesar. Bovine serum albumin (BSA) (A7638), Potassium phospate dibasic trihydrate (60349), 3-(Trimethoxysilyl)propylmethacrylate (440159), 2-Hydroxy-4′-(2-hydroxyyethoxy)-2-methylpropiophenone (410896), Poly(ethylene glycol) diacrylate (PEGDA) 700 Da (455008), l -lactate dehydrogenase (LDH) from muscle rabbit (L1254), Jack bean urease (U4002), β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate (NADH) (N8129), Pyruvate (P8524), Lactate assay Kit (MAK064), Phenol nitroprusside solution (P6994), Alkaline hypochlorite solution (A1727), Urease Activity Assay Kit (MAK120), Rhodamine-B (R6626) were purchased from Sigma-Aldrich. Potassium phosphate monobasic (42420) was purchased from Acros Organics. Deuterium oxide (D2O) (DE50B) was purchased from Apollo. N,N-dimethylformamide (DMF) (D119) was purchased from Fisher Chemicals. Alexa Fluor 594 and 488 Microscale Protein Labeling Kit (A30008 and A30006) and Carboxylic acid, Acetate, Succinimidyl Ester SNARF-1 (S2280) were purchased from Thermo Fischer Scientific. Trichloroacetic acid (TCA) (34603) was purchased from Nacalai Tesque. Fluorescent polystyrene nanoparticles tracers of 0.2 and 1 μm of diameter (FCDG003, FCDG006) were purchased from Bangs Laboratories. Fluorescein isothiocyanate (FITC) (AB178737) was purchased from abcr.
Alkaline hypochlorite solution
Manufactured by Merck Group
Alkaline hypochlorite solution is a laboratory reagent used as an oxidizing agent in various chemical analyses and procedures. It is a clear, colorless to pale yellow liquid with a distinctive chlorine-like odor. The solution contains sodium hypochlorite (NaClO) and sodium hydroxide (NaOH) as the primary active ingredients. This product is typically used in controlled laboratory settings to facilitate specific chemical reactions and processes.
Automatically generated - may contain errors
Lab products found in correlation
Phenol nitroprusside solution, by Merck Group (3 mentions)
Poly ethylene glycol diacrylate, by Merck Group (1 mentions)
Lactate assay kit, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Thermo Fisher Scientific (1 mentions)
3 trimethoxysilyl propyl methacrylate, by Merck Group (1 mentions)
Pyruvate, by Merck Group (1 mentions)
L lactate dehydrogenase, by Merck Group (1 mentions)
β nicotinamide adenine dinucleotide, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Thermo Fisher Scientific (1 mentions)
Reduced disodium salt hydrate, by Merck Group (1 mentions)
Phenol nitroprusside, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Nh4cl, by Merck Group (1 mentions)
4 protocols using alkaline hypochlorite solution
1
Comprehensive Biophysical Characterization of PEGylated Hydrogels
2
Formamidase Activity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
First the protein extracts were incubated with different concentrations of TSC (1 mM, 125 μM, 32 μM and 15 μM) or TSC-C (1 mM, 62 μM, 18 μM and 9 μM).Then, formamidase activity was measured by monitoring the production of ammonia, as previously described [31 (link)]. Protein samples (500 ng) were added to 100 mM formamide, 100 mM phosphate buffer, pH 7.4, and 10 mM EDTA. The reaction mixture was incubated at 37°C for 30 min. Subsequently, 400 mL phenol-nitroprusside and 400 mL alkaline hypochlorite solution (Sigma Aldrich) were added. The samples were incubated at 50°C for 6 min and the enzymatic activity was monitored at 625 nm. The amount of ammonia released was determined through comparison with a standard curve. The specific activity was calculated as the measured absorbance divided by the amount of proteins in μg.
3
Serum BUN Measurement Protocol
4
Quantification of Urease Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The assays used to characterize
urease activity were based on the Berthelot method.60 (link),61 (link) Briefly, in a 96-well plate, reactions of 50 μL of urea solubilized
in 1× PBS at a set concentration were mixed with 50 μL
of soluble urease (diluted to a final concentration of 0.5 nM in 1×
PBS) or particles and incubated for 2 min at RT. Wells containing
0–200 μM ammonium chloride solution (NH4Cl,
Sigma-Aldrich, cat. no. 254134) were prepared alongside the assays
in order to make a calibration curve to quantify ammonium production.
To stop the ureolytic reactions, 80 μL of phenol nitroprusside
solution (Sigma-Aldrich, cat. no. P6994) was added to each assay and
calibration standard, followed by 40 μL of an alkaline hypochlorite
solution (Sigma-Aldrich, cat. no. A1727). The plates were mixed thoroughly,
then incubated for 30 min at 37 °C, before reading the absorbance
of the plate at 630 nm. The rate of the urease reaction was determined
using the NH4Cl calibration curve.
urease activity were based on the Berthelot method.60 (link),61 (link) Briefly, in a 96-well plate, reactions of 50 μL of urea solubilized
in 1× PBS at a set concentration were mixed with 50 μL
of soluble urease (diluted to a final concentration of 0.5 nM in 1×
PBS) or particles and incubated for 2 min at RT. Wells containing
0–200 μM ammonium chloride solution (NH4Cl,
Sigma-Aldrich, cat. no. 254134) were prepared alongside the assays
in order to make a calibration curve to quantify ammonium production.
To stop the ureolytic reactions, 80 μL of phenol nitroprusside
solution (Sigma-Aldrich, cat. no. P6994) was added to each assay and
calibration standard, followed by 40 μL of an alkaline hypochlorite
solution (Sigma-Aldrich, cat. no. A1727). The plates were mixed thoroughly,
then incubated for 30 min at 37 °C, before reading the absorbance
of the plate at 630 nm. The rate of the urease reaction was determined
using the NH4Cl calibration curve.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!