Mercury vx 300 nmr spectrometer

All melting points were measured on a Gallenkamp melting point apparatus and were uncorrected. The infrared spectra were recorded using potassium bromide disks on a Mattson FTIR spectrophotometer (Mattson, New York, NY, USA). ¹H-NMR spectra were run at 300 MHz, on a Varian Mercury VX-300 NMR spectrometer (Bruker, Rheinstetten, Germany) using TMS as an internal standard in deuterated dimethylsulphoxide. ¹³C-NMR spectra were recorded on a Bruker spectrometer at 100 MHz. Chemical shifts δ are quoted in ppm. The mass spectra were recorded on a GCMS-QP-1000EX mass spectrometer (Shimadzu, Kyoto, Japan) at 70 e.V. All the spectral measurements were carried out at the Microanalytical Center of Cairo University, Cairo, Egypt; and the Main Defense Chemical Laboratory, Cairo, Egypt and Zagazig University, Zagazig, Egypt. The elemental analyses were carried out at the Microanalytical Center of Ain Shams University, Cairo, Egypt. The pharmaceutical activity assays were carried out at the Pharmacology Department, Faculty of Pharmacy, EL-Mansoura University, EL-Mansoura, Egypt. All reagents used in this study were commercially available. Ethyl 2-(3-(4-chloro-3-methylphenyl)-6-oxo-5,6-dihydropyridazin-1(4H)-yl)acetate (1) and 2-(3-(4-chloro-3-methylphenyl)-6-oxo-5,6-dihydropyridazin-1(4H)-yl)aceto-hydrazide (3) were prepared by previously reported procedures [25 ].

¹H-NMR spectra of Ch and TMC were investigated using a Varian Mercury (VX-300 NMR) spectrometer (Bruker, Massachusetts, United States) at 300 MHz in dimethyl sulphoxide/trifluoroacetic acid as a control solvent for the Ch sample, while D₂O was the control solvent for TMC.