Ppi 200 programmable power inverter

Manufactured by Bio-Rad

Sourced in United States

The PPI-200 programmable power inverter is a laboratory equipment product designed to convert alternating current (AC) to direct current (DC) power. It provides a stable and adjustable DC output voltage for various laboratory applications.

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Lab products found in correlation

Seakem le agarose gel, by Lonza (1 mentions)

2 protocols using ppi 200 programmable power inverter

Plasmid Composition Analysis via FIGE

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To assess plasmid composition, field inversion gel electrophoresis was performed. Genomic DNA was used from all the newly acquired and previously published Borrelia isolates that were available [12 (link)]. Approximately 500 ng of genomic DNA from each isolate was loaded in a 1% Tris Borate EDTA (TBE) agarose gel and separated using the Owl D4 Horizontal Gel Electrophoresis System (Thermo fisher, Waltham, MA, USA) and a PPI-200 programmable power inverter (MJ Research, Inc., Waltham, MA, USA). The gel was run at 100V for 15 minutes and then run at 90V for ~ 40 hours using program 3 of the PPI-200 programmable power inverter.

Krishnavajhala A., Armstrong B.A., Kneubehl A.R., Gunter S.M., Piccione J., Kim H.J., Ramirez R., Castro-Arellano I., Roachell W., Teel P.D, & Lopez J.E. (2021). Diversity and distribution of the tick-borne relapsing fever spirochete Borrelia turicatae. PLoS Neglected Tropical Diseases, 15(11), e0009868.

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Plasmid DNA Electrophoresis Separation

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Plasmid DNA (500 ng) was electrophoresed on a 0.7% SeaKem LE Agarose gel (Lonza, Basel, Switzerland) with 0.5× TBE buffer. DNA was initially electrophoresed without inversion at 100 V for 15 min, followed by 24 hr of inversion with recirculation of buffer at 4°C. The reverse‐pulse electrical field was supplied by a PPI‐200 Programmable Power Inverter (MJ Research, Watertown, MA) on Program 3.

James A.E., Rogovskyy A.S., Crowley M.A, & Bankhead T. (2017). Cis‐acting DNA elements flanking the variable major protein expression site of Borrelia hermsii are required for murine persistence. MicrobiologyOpen, 7(3), e00569.

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