10‐week‐old female C57BL/6 mice were purchased from Envigo (UK). Tcell reporter of activation and cell enumeration (TRACE) and C57BL/6 mice were maintained at the University of Glasgow under specific pathogen‐free conditions in accordance with UK home office regulations (Project Licence P2F28B003) and approved by the local ethics committee.
To generate mice in which rtTA reports interleukin‐2 (IL‐2) expression, we used recombineering to extract the upstream 8.389 kb section of the IL‐2 promoter from a Bacterial Artificial Chromosome (BAC) RP24208L3 (BAC resource at Children's Hospital Oakland Research Institute, Buffalo, New York). This was subcloned into a plasmid containing the human CD2 locus control region and linked to the rtTA sequence. The transgene, cut and purified from the construct backbone, was used to create transgenic mice at the Transgenic mouse facility at National Jewish Health in Jackson Lab C57BL/6 animals. Two founder pups were identified by PCR, but only one was fertile. Progeny of this animal were bred with B6.Cg‐Tg(tetO‐cre)1Jaw/J (006234) and B6.129X1‐Gt(ROSA)26Sortm1(EYFP+)Cos (006148) both from Jackson Laboratories.
To generate mice in which rtTA reports interleukin‐2 (IL‐2) expression, we used recombineering to extract the upstream 8.389 kb section of the IL‐2 promoter from a Bacterial Artificial Chromosome (BAC) RP24208L3 (BAC resource at Children's Hospital Oakland Research Institute, Buffalo, New York). This was subcloned into a plasmid containing the human CD2 locus control region and linked to the rtTA sequence. The transgene, cut and purified from the construct backbone, was used to create transgenic mice at the Transgenic mouse facility at National Jewish Health in Jackson Lab C57BL/6 animals. Two founder pups were identified by PCR, but only one was fertile. Progeny of this animal were bred with B6.Cg‐Tg(tetO‐cre)1Jaw/J (006234) and B6.129X1‐Gt(ROSA)26Sortm1(EYFP+)Cos (006148) both from Jackson Laboratories.