Human apoa 1

All reagents and solvents were LC–MS grade quality unless otherwise noted. The gene for Human ApoA-1 in the Gateway entry vector pFN21AE0099, FuGENE^® 6 transfection reagent, protease inhibitors, and mammalian cell lysing buffer purchased from Promega (Madison, WI, USA). Human ApoA-1 isolated from human HDL was from ProSpec (East Brunswick, NJ, USA). Mouse serum was from Sigma-Aldrich (St. Louis, MO, USA). SILAC amino acids including [¹³C₆¹⁵N₂]-lysine and [¹³C₉¹⁵N₁]-tyrosine were from Cambridge Isotope Laboratories (Andover, MA, USA). DL-dithiothreitol (DTT), iodoacetamide (IAA) and dialyzed fetal bovine serum was from Sigma-Aldrich. 2-mecaptoethanol (BME) was from BioRad (Hercules, CA, USA). Ammonium bicarbonate (ABC) and formic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA). Colloidal Coomassie and sequencing-grade modified trypsin from Promega. LC grade water and acetonitrile were from Burdick and Jackson (Muskegon, MI, USA). Custom-made AQUA peptides corresponding to endogenous peptides of trypsin-digested ApoA-1 were purchased from Thermo Scientific (Isotopic enrichment >99%, HeavyPeptide AQUA custom synthesis service (Rockford, IL, USA).