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Recombinant human fgf basic 100 18b

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Recombinant Human FGF-basic (100-18B) is a laboratory product manufactured by Thermo Fisher Scientific. It is a recombinant form of the human basic fibroblast growth factor (FGF-basic or FGF2), a signaling protein involved in various cellular processes. The product is provided as a lyophilized powder.

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Lab products found in correlation

Pyruvate solution, by Thermo Fisher Scientific (2 mentions) Doxycycline hyclate, by Thermo Fisher Scientific (2 mentions) Tamoxifen, by Roche (2 mentions) Protein block, by Agilent Technologies (2 mentions) Betazoid dab chromogen kit, by Biocare Medical (2 mentions) Cyquant nf, by Thermo Fisher Scientific (2 mentions) 2 deoxyglucose, by Roche (2 mentions) Sodium oxamate, by Roche (2 mentions) Sodium oleate, by Roche (2 mentions) Mouse egf 354001, by Bio-Techne (2 mentions) 1 14c oleic acid, by PerkinElmer (2 mentions) Kin112, by Biocare Medical (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Rock inhibitor y 27632, by Merck Group (2 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (2 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) 4 bromocrotonic acid brca, by Tokyo Chemical Industry (2 mentions) Matrigel, by Corning (2 mentions)

3 protocols using recombinant human fgf basic 100 18b

1

Stem Cell Differentiation Protocol

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Laminin (23017-95), CyQUANT NF (C35006), bovine pituitary extract (13038-14), HBSS, RPMI, DMEM, DMEM:F12, penicillin-streptomycin, pyruvate solution, fetal bovine serum (FBS), BODIPY 493/503 (D3922), B-27™ Supplement (50X), serum free (17504044) were from Life Technologies; Recombinant Human FGF-basic (100-18B) from Peprotech; doxycycline hyclate (D9891), nicotinamide (N3376), dexamethasone (D4902), 2-deoxyglucose (D6134), Sodium oxamate (O2751), collagenase (C7657), glucose (G7528), tamoxifen (T5648), Sodium oleate (O7501), Bovine Serum Albumin (A7030), Alcian Blue (B8438), Roche cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail (11836170001), ROCK inhibitor: Y-27632 (SCM075) were from Sigma;, Forskolin (1099), IBMX (2845), KT5720 (1288), WWL113 (5259) were from Tocris; mouse EGF (354001), NuSerum IV (355104), ITS+ Premix (354352) and Matrigel (354234) were from Corning; dispase (165–859; Roche); Sp-8-Br-cAMPS (B002 ) and 8-pCPT-2′-O-Me-cAMP (C041) were from Biology Life Science Institute; Oleic acid-[1-14C] was from Perkin-Elmer (NEC315070UC); 4-Bromocrotonic Acid (BrCA) (B2298) was from TCI America; iTaq universal SyBR Green Supermix (BIO-RAD), Protein Block (X0909; DAKO); MACH2 HRP-Polymer (HRP520H) and Betazoid DAB Chromogen Kit (BDB2004H) from Biocare Medical; HG-9-91-01 and KIN112 were kindly provided by Dr. Nathanael Gray.
Patra K.C., Kato Y., Mizukami Y., Widholz S., Boukhali M., Revenco I., Grossman E.A., Ji F., Sadreyev R.I., Liss A.S., Screaton R.A., Sakamoto K., Ryan D.P., Mino-Kenudson M., Castillo C.F., Nomura D.K., Haas W, & Bardeesy N. (2018). Mutant GNAS drives pancreatic tumorigenesis by inducing PKA-mediated SIK suppression and reprogramming lipid metabolism. Nature cell biology, 20(7), 811-822.
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2

Sphere Formation of HCC Cells

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5 × 104 HCC cells were placed in ultra-low binding 6-well dishes and grown in DMEM/F12 medium. Animal-Free Recombinant Human EGF (AF-100-15, Peprotech), Recombinant Human FGF-basic (100-18B, Peprotech) and N-2 supplement (17502-048, ThermoFisher) were added to the medium to ensure cell sphere formation. When the spheres formed by the cells grew to 8 days, they were viewed and photographed using a microscope.
Wu Q., Qiu Y., Guo J., Yuan Z., Yang Y., Zhu Q., Zhang Z., Guo J., Wu Y., Zhang J., Huang D., Tu K, & Hu X. (2024). USP40 promotes hepatocellular carcinoma cell proliferation, migration and stemness by deubiquitinating and stabilizing Claudin1. Biology Direct, 19, 13.
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3

Stem Cell Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Laminin (23017-95), CyQUANT NF (C35006), bovine pituitary extract (13038-14), HBSS, RPMI, DMEM, DMEM:F12, penicillin-streptomycin, pyruvate solution, fetal bovine serum (FBS), BODIPY 493/503 (D3922), B-27™ Supplement (50X), serum free (17504044) were from Life Technologies; Recombinant Human FGF-basic (100-18B) from Peprotech; doxycycline hyclate (D9891), nicotinamide (N3376), dexamethasone (D4902), 2-deoxyglucose (D6134), Sodium oxamate (O2751), collagenase (C7657), glucose (G7528), tamoxifen (T5648), Sodium oleate (O7501), Bovine Serum Albumin (A7030), Alcian Blue (B8438), Roche cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail (11836170001), ROCK inhibitor: Y-27632 (SCM075) were from Sigma;, Forskolin (1099), IBMX (2845), KT5720 (1288), WWL113 (5259) were from Tocris; mouse EGF (354001), NuSerum IV (355104), ITS+ Premix (354352) and Matrigel (354234) were from Corning; dispase (165–859; Roche); Sp-8-Br-cAMPS (B002 ) and 8-pCPT-2′-O-Me-cAMP (C041) were from Biology Life Science Institute; Oleic acid-[1-14C] was from Perkin-Elmer (NEC315070UC); 4-Bromocrotonic Acid (BrCA) (B2298) was from TCI America; iTaq universal SyBR Green Supermix (BIO-RAD), Protein Block (X0909; DAKO); MACH2 HRP-Polymer (HRP520H) and Betazoid DAB Chromogen Kit (BDB2004H) from Biocare Medical; HG-9-91-01 and KIN112 were kindly provided by Dr. Nathanael Gray.
Patra K.C., Kato Y., Mizukami Y., Widholz S., Boukhali M., Revenco I., Grossman E.A., Ji F., Sadreyev R.I., Liss A.S., Screaton R.A., Sakamoto K., Ryan D.P., Mino-Kenudson M., Castillo C.F., Nomura D.K., Haas W, & Bardeesy N. (2018). Mutant GNAS drives pancreatic tumorigenesis by inducing PKA-mediated SIK suppression and reprogramming lipid metabolism. Nature cell biology, 20(7), 811-822.
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