Immunohistochemistry staining were performed as described previously [13] (link). The primary rabbit anti-RUNX3 antibody (1∶200) (Medical and Biological Laboratories, Nagoya, Japan), anti-VIII (1∶100), anti-PAP (1∶100) (Santa Cruz, CA, USA) and the biotinylated anti-mouse IgG (1∶200) (Boster Biotech, Wuhan, China) were used. For the TMA staining evaluation, the immunoreactivity was assessed blindly by two independent observers using light microscopy (Olympus BX-51 light microscope), and the image was collected by Camedia Master C-3040 digital camera. The expression of RUNX3 was graded as positive when 10% of tumor cells showed immunopositivity. Biopsies with 10% tumor cells showing immunostaining were considered negative [15] (link).
Anti 8
Manufactured by Santa Cruz Biotechnology
Sourced in Japan
Anti-VIII is a laboratory reagent used for the detection and quantification of Factor VIII (FVIII) in biological samples. It is designed to provide accurate and reliable results in research and diagnostic applications involving FVIII analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti 8
1
Immunohistochemical Evaluation of RUNX3 Expression
2
Ischemic Brain Vascular Morphology and BVECs Apoptosis
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Vascular morphology in ischemic brain was observed by the immunohistochemistry stain of CD31, and BVECs apoptosis was visualized by VIII/TUNEL/DAPI stain. Rats were sacrificed for brain sections on the 4th day. 20 μm frozen sections and 4 μm paraffin sections of brains were prepared for immunofluorescence and immunohistochemistry respectively. Specific protocols were conducted according to previous reports 23 . Primary antibodies were anti-VIII (1:300 Santa Cruz sc3657) and anti-CD31 (1:300 Sigma-Aldrich Inc.).
The vessel morphology in the right cerebral cortex of coronal sections (1 mm, 2 mm, and 3 mm from optical chiasm) was observed. Those areas were selected because they are damaged by MCAO. The number of degraded vessel and the number of apoptotic BVECs were counted in three regions at size of 500 × 500 μm2 in each section.
The vessel morphology in the right cerebral cortex of coronal sections (1 mm, 2 mm, and 3 mm from optical chiasm) was observed. Those areas were selected because they are damaged by MCAO. The number of degraded vessel and the number of apoptotic BVECs were counted in three regions at size of 500 × 500 μm2 in each section.
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