Phosphatidylinositol specific phospholipase c

Manufactured by Merck Group

Sourced in United States, Netherlands

Phosphatidylinositol-specific phospholipase C is an enzyme that catalyzes the hydrolysis of phosphatidylinositol and its phosphorylated derivatives. It plays a role in the regulation of cellular signaling pathways.

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Lab products found in correlation

Alexa fluor 647 conjugated anti mouse cd106 clone 429, by BioLegend (1 mentions) Pe conjugated anti mouse upar clone 109801, by R&D Systems (1 mentions) Pe conjugated hamster anti mouse cd55, by BD (1 mentions) Anti mouse cd59a clone 7a6, by BD (1 mentions) Montage antibody purification kit, by Merck Group (1 mentions) Pierce fab preparation kit, by Thermo Fisher Scientific (1 mentions) Egfr inhibitor ag1478, by Merck Group (1 mentions) Sb203580, by Merck Group (1 mentions) Gm6001, by Merck Group (1 mentions) Peroxidase conjugated anti rabbit igg antibody, by Jackson ImmunoResearch (1 mentions) Dulbecco s minimal essential medium dmem, by Corning (1 mentions) ε aminocaproic acid eaca, by Merck Group (1 mentions) Total erk 1 2 antibody, by BD (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Corning (1 mentions) Pd98059, by Merck Group (1 mentions) Sp600125, by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions)

Spelling variants of this product

Phosphatidylinositol (14 citations) Phospholipase C (12 citations) Phospholipase C phosphatidylinositol-specific (PI-PLC) from Bacillus cereus (2 citations)

3 protocols using phosphatidylinositol specific phospholipase c

Enzymatic Release of GPI-Anchored Proteins

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The enzymatic action of phosphatidylinositol-specific phospholipase C (Sigma-Aldrich, St Louis, MO, USA) results in the release of GPI-anchored proteins, such as VCAM-1, uPAR, CD55 and CD59, from the cell membranes. BM stromal cells and BM mononuclear cells (MNCs) were incubated with phosphatidylinositol-specific phospholipase C (5 or 100 mU/ml) for 3 h at 37 °C. Alexa Fluor 647-conjugated anti-mouse CD106 clone 429 (BioLegend, San Diego, CA, USA), PE-conjugated anti-mouse uPAR clone 109801 (R&D Systems, Minneapolis, MN, USA), PE-conjugated hamster anti-mouse CD55 and anti-mouse CD59a clone 7A6 (BD Biosciences, San Jose, CA, USA) were applied to detect GPI-anchored proteins.

Adamiak M., Poniewierska-Baran A., Borkowska S., Schneider G., Abdelbaset-Ismail A., Suszynska M., Abdel-Latif A., Kucia M., Ratajczak J, & Ratajczak M.Z. (2015). Evidence that a lipolytic enzyme—hematopoietic-specific phospholipase C-β2—promotes mobilization of hematopoietic stem cells by decreasing their lipid raft-mediated bone marrow retention and increasing the promobilizing effects of granulocytes. Leukemia, 30(4), 919-928.

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Purification and Characterization of Prion Protein Antibodies

Cited 1 time

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POM1, POM3, POM4, POM6 and POM11 antibodies (Polymenidou et al., 2008 (link)) were provided to J.T. by the University of Zürich, Institute of Neuropathology. Hybridomas producing the human-mouse chimeric monoclonal antibodies D13 and D18 (Safar et al., 2002 (link); Williamson et al., 1998 (link)) were provided by Anthony Williamson, Dennis Burton, and Bruce Chesebro. Antibodies were affinity-purified using protein A spin columns (Montage Antibody Purification Kit, EMD Millipore). Fab fragments of D18 were prepared using the Pierce Fab preparation kit from Thermo Scientific. The purity of all antibody preparations was verified by SDS-PAGE. The following antibodies were purchased from commercial sources: 100B3 (Wagening UR, Netherlands); 6D11 (BioLegend, cat #808001); ICSM-18 and ICSM-35 (D-Gen Ltd.).
Pentosan polysulfate (average MW = 4500–5000) was purchased from Biopharm Australia Pty Ltd., and phosphatidylinositol-specific phospholipase C from Sigma (cat #P5542).

Wu B., McDonald A.J., Markham K., Rich C.B., McHugh K.P., Tatzelt J., Colby D.W., Millhauser G.L, & Harris D.A. (2017). The N-terminus of the prion protein is a toxic effector regulated by the C-terminus. eLife, 6, e23473.

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Inhibition of MAPK Signaling Pathways

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suPAR and ATF were purchased from American Diagnostica, Inc. (Greenwich, CT). fMLP, Pertussis toxin (Gαi inhibitor, 100ng/ml), Phosphatidylinositol-specific-phospholipase C (100µg/mL), PD98059 (ERK inhibitor, 25µM) SB203580 (p38^MAPK inhibitor, 10µM), SP600125 (JNK inhibitor, 1µM), EGFR inhibitor ( AG1478, 10µM), ε-aminocaproic acid (EACA; plasmin inhibitor, 100µM), aprotinin (plasmin inhibitor, 100 units/ml) and GM6001 (MMP inhibitor, 10nM) were purchased from Sigma Chemical Co. (St. Louis, MO). Peroxidase-conjugated anti-rabbit IgG antibody (raised in goat) and peroxidase-conjugated anti-mouse IgG antibody (raised in goat) were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA). Phospho-ERK1/2 antibody was purchased from Promega, Inc. (Madison, WI). Total ERK 1/2 antibody was purchased from BD Transduction Laboratories (Lexington, KY). Phospho-p38^MAPK and phospho-JNK antibodies were purchased from Biosource (Camarillo, CA). Total p38^MAPK and JNK antibodies were purchased from Cell Signaling (Beverley, MA). Dulbecco’s minimal essential medium (DMEM) and Dulbecco’s phosphate buffered saline (dPBS) were purchased from Corning Cellgro (Corning, NY).

Duru E.A., Fu Y, & Davies M.G. (2015). ROLE OF FORMIC RECEPTORS IN SOLUBLE UROKINASE RECEPTOR INDUCED HUMAN VASCULAR SMOOTH MUSCLE MIGRATION. The Journal of surgical research, 195(2), 396-405.

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