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Cat diagnostic kits

Manufactured by Solarbio
Sourced in China

The CAT diagnostic kits provided by Solarbio are designed for the quantitative determination of catalase (CAT) activity in various biological samples. The kits utilize a colorimetric method to measure the catalase enzyme's ability to decompose hydrogen peroxide (H2O2) into water and oxygen. The core function of these kits is to provide a reliable and accurate assessment of catalase levels, which is essential for various research and clinical applications.

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2 protocols using cat diagnostic kits

1

Antioxidant Pathway Activation Assay

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H2O2 was purchased from HUSHI (HUSHI, Shanghai, China). CCK-8, NAC were purchased from Beyotime (Beyotime, Shanghai, China). PCs, 2′,7′-dichlorofluorescin diacetate, MDA, GSH-Px, SOD diagnostic kits, and CAT diagnostic kits were purchased from Solarbio (Solarbio, Beijing, China). RNAiso Plus, PrimeScript RT reagent Kit with gDNA Eraser, and SYBR Premix Ex Taq II were purchased from Takara (Takara, Shiga, Japan). Nrf2-siRNA, control-siRNA, and lipofectamine 2000 were obtained from GenePharma (GenePharma, Shanghai, China). The following primary antibodies and corresponding secondary antibodies were obtained from Proteintech (Proteintech, Wuhan, China): Nrf2, HO-1, GCLC, GCLM, NAD(P)H: NQO1, lamin B, and GAPDH.
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2

Nrf2-Mediated Oxidative Stress Responses

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MPP+ and MPTP agents were purchased from Sigma (St. Louis, MO, USA), and the Cell Counting Kit-8 was obtained from Beyotime (Shanghai, China). The PCs, 2′,7′-Dichlorofluorescin diacetate, MDA, GSH-Px, SOD and CAT diagnostic kits were all obtained from Solarbio (Beijing, China). The RNAiso Plus, PrimeScript™RT Reagent Kit with gDNA Eraser, and SYBR®Premix Ex Taq™ II were purchased from Takara (Shiga, Japan); the Nrf2-siRNA, control-siRNA, and Lipofectamine 2000 were obtained from GenePharma (Shanghai, China). Finally, the primary antibodies, Nrf2, HO-1, GCLC, GCLM, NAD(P)H: NQO1, Lamin B, GAPDH, Antityrosine Hydroxylase (TH) and corresponding secondary antibodies were obtained from Proteintech (Wuhan, China).
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