Anti phospho p38 and p38 antibodies

LPS (Escherichia coli serotype O127:B8) was from Sigma Aldrich (Allentown, PA, USA). ATP disodium salt and phorbol 12-myristate 13-acetate (PMA) were from MedChemExpress (Monmouth Junction, NJ, USA). A stock solution of LXA₄ (Cayman Chemical, Ann Arbor, MI, USA) was stored at −80 °C until being diluted in sterile 0.9% saline or cell culture medium immediately before use. Anti-NF-κB p65, -ALOX12, -ALOX15, -ALOX15B, -AT1R, -β-actin, and -Lamin B antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti phospho-P38 and P38 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-Caspase-1 antibody was from Abcam Company (Cambridge, UK). Anti-CD68 antibody was from Proteintech Group, Inc. (Wuhan, China). APC-conjugated Rat Anti-Mouse CD19 and PE-conjugated Rat Anti-Mouse CD5 were from BD Biosciences (San Diego, CA, USA). Mouse ALOX5 monoclonal antibody (BD Biosciences) was used for western blotting (WB), whereas rabbit ALOX5 polyclonal antibody (Cayman Chemical) was used for immunohistochemistry (IHC). RIPA Lysis Buffer and Nuclear and Cytoplasmic Protein Extraction Kit were from Beyotime Institute of Biotechnology (Shanghai, China). BCA protein assay kit was from Pierce (Rockford, IL, USA).

The reagents used in this study were mostly obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-CD41(α_IIb) and anti-CD62 (P-selectin) antibodies tagged to fluorescein isothiocyanate (FITC) were obtained from BD Bioscience (San Jose, CA, USA). Phycoerythrin-tagged antibodies that recognize the active form of murine α_IIbβ₃ (JON/A) were purchased from Emfret Analytics (Eibelstadt, Germany). Thrombin was brought from Hematologic Technologies Inc. (Essex Junction, VT, USA). ADP and collagen were purchased from Helena Laboratories (Beaumont, TX, USA). Thromboxane analog (U44619) and water-soluble p38 SB203580 hydrochloride were ordered from Bio-Techne Corporation (Minneapolis, MN, USA). Fibrinogen and Alexa 488-tagged fibrinogen were purchased from Enzyme Research Laboratories Inc. (South Bend, IN, USA) and Invitrogen, (Carlsbad, CA, USA), respectively. Anti-phospho-p38 and p38 antibodies were obtained from Cell Signaling (Boston, MA, USA).