Anti nrf2 ab137550

Manufactured by Abcam

Sourced in United States

Anti-NRF2 (ab137550) is a rabbit monoclonal antibody that recognizes the Nuclear factor erythroid 2-related factor 2 (NRF2) protein. NRF2 is a transcription factor that regulates the expression of antioxidant proteins and enzymes.

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Lab products found in correlation

Gapdh sc 25778, by Santa Cruz Biotechnology (2 mentions) Anti nedd8 ab81264, by Abcam (1 mentions) Anti γ h2a x ab81299, by Abcam (1 mentions) Anti cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions) Anti abcg2 sc 58222, by Santa Cruz Biotechnology (1 mentions) Fumitremorgin c, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Mln4924, by Cayman Chemical (1 mentions) Anti ho 1 ab68477, by Abcam (1 mentions) Ab68477, by Abcam (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-Nrf2 (161 citations) Ab137550 (134 citations) Nrf2 (ab137550) (2 citations)

4 protocols using anti nrf2 ab137550

ABCG2 Inhibitor Screening Protocol

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Reagents were obtained from: MLN4924 (Cayman Chemical, Ann Arbor, MI), YHO-13351 and fumitremorgin C (FTC), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI) (Sigma, St. Louis, MO, USA). Antibodies were purchased from the following sources: (anti-NEDD8 [ab81264], anti-γ-H2AX [ab81299], anti-NRF2 [ab137550], and anti-p27 [ab32034] (Abcam, Cambridge, MA, USA), anti-p21 [05-345] (EMD Millipore, Burlington, MA, USA), anti-cleaved caspase-3 [9661] (Cell Signaling Technology, Danvers, MA, USA), anti-β-tubulin [T7816] (Sigma), anti-ABCG2 [sc-58222] (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and sheep anti-mouse-horseradish peroxidase (HRP) and donkey anti-rabbit-HRP (Amersham, Pittsburgh, PA, USA).

Kathawala R.J., Espitia C.M., Jones T.M., Islam S., Gupta P., Zhang Y.K., Chen Z.S., Carew J.S, & Nawrocki S.T. (2020). ABCG2 Overexpression Contributes to Pevonedistat Resistance. Cancers, 12(2), 429.

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Ginsengenin-S2 Modulates HDAC2 and Nrf2 in CSE-Induced Cells

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Western blot assay was performed as previous described [6 (link)] to study the effects of ginsengenin-S2 on the protein expression of HDAC2 and Nrf2 in CSE-stimulated A549 cells. Anti-HDAC2 (#ab32117), anti-Nrf2 (#ab137550) and anti-β-actin (#ab137550) were from Abcam Company (Cambridge, UK). The HDAC2 and Nrf2 band intensities were adjusted with reference to β-actin control.

Qi Z., Li Z., Guan X., Wang C., Wang F., Li P, & Liu J. (2019). Four Novel Dammarane-Type Triterpenoids from Pearl Knots of Panax ginseng Meyer cv. Silvatica. Molecules, 24(6), 1159.

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Medicarpin from Robinia pseudoacacia root

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Medicarpin was isolated from the root of Robinia pseudoacacia L. obtained in Korea, and the chemical structure was identified by comparing the EI-MS, ¹H-, and ¹³C-NMR spectroscopic data with the published ones. Anti-NRF2 (ab137550) and anti-HO-1 (ab68477) antibodies were obtained from Abcam (Cambridge, MA, USA). Antibodies against Lamin A/C, GFP, and GAPDH (sc-25778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Kim J.H., Kang D.M., Cho Y.J., Hyun J.W, & Ahn M.J. (2022). Medicarpin Increases Antioxidant Genes by Inducing NRF2 Transcriptional Level in HeLa Cells. Antioxidants, 11(2), 421.

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Antioxidant Activity of Camellia japonica Root Extract

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C. japonica roots (CJRE) were collected in the southwest (Haenam, Jeollanam-do) of South Korea in August 2016. The dried C. japonica roots (11.5 kg) were chopped into 3–5 cm pieces and were extracted with 80% MeOH for 3 h twice using an ultrasonic apparatus. The methanolic extract of CJRE (476 g) was yielded after evaporating in vacuo and freeze-dried as powder. Anti-NRF2 (ab137550) and anti-HO-1 (ab68477) antibodies were obtained from Abcam (Cambridge, MA). Antibodies against phospho-AKT (#4060), and AKT (#4691), phospho-p38(#9211), p38 (#9212), phospho-pERK1/2 (#4377), pERK1/2 (#4696), were purchased from Cell Signaling Technology (Danvers, MA). Antibodies against Lamin A/C, GFP, beta-Actin, and GAPDH (sc-25778) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Kim J.H., Yang H, & Kim K.K. (2022). Camellia japonica Root Extract Increases Antioxidant Genes by Induction of NRF2 in HeLa Cells. Plants, 11(21), 2914.

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