Rhfsh

Manufactured by Promega

Sourced in United States

RhFSH is a recombinant human follicle-stimulating hormone (rh-FSH) product. It is a glycoprotein hormone that plays a key role in the regulation of the reproductive system. RhFSH is used in various assisted reproductive technology (ART) procedures.

Automatically generated - may contain errors

Lab products found in correlation

α mem, by Thermo Fisher Scientific (2 mentions) Activin a, by Merck Group (2 mentions) Rhfsh, by Merck Group (2 mentions) Rhegf, by Promega (2 mentions) Insulin, by Merck Group (1 mentions) Transferrin, by Merck Group (1 mentions) Selenium, by Merck Group (1 mentions) Ksom aa, by Merck Group (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Selenium, by Thermo Fisher Scientific (1 mentions) Transferrin, by Thermo Fisher Scientific (1 mentions)

2 protocols using rhfsh

Ovarian Tissue Culture and IVF Protocol

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Ovarian tissue culture was performed using in vitro growth (IVG) medium consisting of αMEM (Invitrogen, CA) supplemented with 5% FBS, 100 mIU/ml rhFSH (MERCK, NJ), 5 μg/ml insulin, 5 μg/ml transferrin, 5 ng/ml selenium (Sigma-Aldrich, MO). Activin A (Sigma-Aldrich, MO) was used as an IVG supplement (30 ng/ml). IVM medium consisted of αMEM supplemented with 5% FBS, 100 mIU/ml rhFSH and 5 ng/ml rhEGF (Promega, WI). TYH medium was used for IVF. After IVF, fertilized oocytes and embryos were cultured in mCZB medium.

Higuchi C.M., Maeda Y., Horiuchi T, & Yamazaki Y. (2015). A Simplified Method for Three-Dimensional (3-D) Ovarian Tissue Culture Yielding Oocytes Competent to Produce Full-Term Offspring in Mice. PLoS ONE, 10(11), e0143114.

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Ovarian tissue culture for in vitro growth

Cited 1 time

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Ovaries were dissected in Hepes- αMEM (Invitrogen, Waltham, MA, USA) supplemented with 5% fetal bovine serum (FBS; Hyclone Laboratories, Logan, UT, USA). Ovarian tissue culture was performed using in vitro growth (IVG) medium as previously described [53 (link)]. αMEM (Invitrogen) was supplemented with 5% FBS, 100 mIU/mL rhFSH (Merck, Kenilworth, NJ, USA), 5 μg/mL insulin, 5 μg/mL transferrin, and 5 ng/mL selenium (Gibco, Carlsbad, CA, USA). According to the culture conditions, activin A (Sigma-Aldrich, St. Louis, MO, USA) was supplemented in the IVG medium (30 ng/mL). The IVM medium consisted of αMEM supplemented with 5% FBS, 100 mIU/mL rhFSH, and 5 ng/mL rhEGF (Promega, Madison, WI, USA). For dissecting the tissues, αMEM-HEPES supplemented with 5% FBS was prepared. HTF (Millipore Sigma, Burlington, MA, USA) was used for IVF. After IVF, fertilized oocytes and embryos were cultured in KSOM-AA (Millipore Sigma).

Matsushige C., Xu X., Miyagi M., Zuo Y.Y, & Yamazaki Y. (2022). RGD-modified dextran hydrogel promotes follicle growth in three-dimensional ovarian tissue culture in mice. Theriogenology, 183, 120-131.

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