E 3 hexen 1 ol

The following compounds (food grade, purity in parenthesis) were purchased from Sigma-Aldrich (St. Louis, MO, USA): ethyl acetate (≥99%), ethanol (96%), hexanal (≥97%), 4-methylpentan-2-ol (98%), E-2-hexenal (≥95%), hexan-1-ol (≥98%), E-3-hexen-1-ol (97%), E,E-2,4-hexadienal (95%), nonanal (≥98%), acetic acid (≥99.5%), E-2-decenal, pentanoic acid (≥99%), hexanoic acid (≥98%), and E,E-2,4-decadienal (≥89%).

The external C6 volatile standards, including hexanal (98.0% purity), (E)-2-hexenal (98.0%), 1-hexanol (99.0%), (E)-2-hexen-1-ol (96.0%), (E)-3-hexen-1-ol (98.0%), (Z)-2-hexen-1-ol (96.0%), (Z)-3-hexen-1-ol (98.0%), ethyl hexanoate (99.0%), hexyl acetate (99.0%), (Z)-3-hexen-1-acetate (98.0%), and hexanoic acid (99.0%), were purchased from Sigma-Aldrich (St. Louis, MO, USA). The internal standard 4-methyl-2-pentanol was also purchased from Sigma-Aldrich, with a purity of 98.0%. Water used in this study was purified from a Milli-Q purification system (Millipore, Bedford, MA, USA). Polyvinylpolypyrrolidone (PVPP) was a product of Sigma-Aldrich. SYBR^® Premix Ex TaqTM and Spectrum™ Plant Total RNA Kit were purchased from TaKaRa Bio (Otsu, Shiga, Japan) and Sigma-Aldrich, respectively. A reverse transcription system kit was obtained from Promega (Madison, WI, USA). Other reagents used in this study were purchased from the Beijing Chemical Works (Beijing, China).