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Model 2200 stimulus isolator

Manufactured by A-M Systems
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Sourced in United States

The Model 2200 stimulus isolator is a laboratory instrument designed to provide controlled electrical stimulation for various research applications. It generates isolated electrical pulses or currents that can be used to stimulate tissues, neurons, or other biological preparations. The device offers adjustable stimulus parameters, including pulse amplitude, duration, and frequency, allowing researchers to fine-tune the stimulation parameters to their specific needs. The Model 2200 is a versatile tool for researchers working in the fields of neurophysiology, electrophysiology, and related areas.

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Lab products found in correlation

Interface chamber, by Fine Science Tools (1 mentions) Model 1800 amplifier, by A-M Systems (1 mentions) Tc 324b temperature controller, by Warner Instruments (1 mentions) Clampex software, by Molecular Devices (1 mentions)

Close spelling variants of this product

Analog stimulus isolator Model 2200 Model 2200 isolator Model 2200

2 protocols using model 2200 stimulus isolator

1

Extracellular LTP Recording Protocol

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LTP experiments were performed using an interface chamber (Fine Science Tools). Oxygenated ACSF (95%/5% O2/CO2; 120 mM NaCl, 2.5 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 1.25 mM NaH2PO4, 25 mM NaHCO3, and 25 mM glucose) warmed to 30°C (TC-324B temperature controller, Warner Instruments) was perfused into the chamber at 1 ml/min. Electrophysiological traces were amplified (Model 1800 amplifier, A-M Systems), digitized and stored (Digidata models 1322A with Clampex software, Molecular Devices). Extracellular stimuli were administered (Model 2200 stimulus isolator, A-M Systems) on the border of area CA3 and CA1 along the Schaffer-collaterals using enameled, bipolar platinum-tungsten (92%:8%) electrodes. fEPSPs were recorded in stratum radiatum with an ACSF-filled glass recording electrode (1–3 MΩ). The relationship between stimulus intensity and fEPSP slopes over various stimulus intensities (0.5–15 V, 25 nA to 1.5 µA) was used to assess baseline synaptic transmission. High-frequency stimulus-induced LTP was induced by administering three 100-Hz tetani (1-s duration) at an interval of 20 s. Synaptic efficacy was monitored 20 min before and 1–3 h following induction of LTP by recording fEPSPs every 20 s (traces were averaged for every 2-min interval).
Laszczyk A.M., Nettles D., Pollock T.A., Fox S., Garcia M.L., Wang J., Quarles L.D, & King G.D. (2019). FGF-23 Deficiency Impairs Hippocampal-Dependent Cognitive Function. eNeuro, 6(2), ENEURO.0469-18.2019.
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2

Electrophysiological Recording of Excitatory Synaptic Currents

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Excitatory postsynaptic currents were recorded from cell bodies identified at 80 × on an AxioExaminer D1 Differential Interference Contrast (DIC) microscope (Zeiss, Thornwood, New York, USA). CA1 neurons were identified by triangular appearance and large apical dendrite. Striatal medium spiny neurons (MSNs) were identified by their oval shape, multiple dendrites, and hyperpolarized resting membrane potential (approximately −85mV) measured after break-in. Stimulating electrode was placed just below the corpus callosum (Fig. 10A) ~150–200µm from the recorded MSN as described [Jaramillo et al., 2016 (link)]. Borosilicate glass electrodes (4–6MX) were filled with internal solution containing (in mM): 110 CsMethanesulfonate, 15 CsCl, 8 NaCl, 10 TEA-Cl, 2 EGTA, 10 HEPES, 3 QX 314, 2 ATP, 0.3 GTP. Observed junction potential was ~10 mV and was compensated. Cells with > 25% change in access resistance (15–25 MΩ) or holding current were not included. Stimulation was controlled via a Model 2200 stimulus isolator (A-M Systems, Sequim, WA, USA) though a monopolar tungsten microelectrode (FHC, Bowdoin, ME) with a 0.1 msec biphasic pulse. 100 µM picrotoxin was in bath for all experiments. Sample size (n) indicates number of cells with no more than five cells per mouse and five or more mice per genotype.
Jaramillo T.C., Speed H.E., Xuan Z., Reimers J.M., Escamilla C.O., Weaver T.P., Liu S., Filonova I, & Powell C.M. (2016). Novel Shank3 Mutant Exhibits Behaviors With Face Validity for Autism and Altered Striatal and Hippocampal Function. Autism research : official journal of the International Society for Autism Research, 10(1), 42-65.
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