Il 2 clone jes6 5h4

Manufactured by BioLegend

IL-2 (clone JES6-5H4) is a purified rat anti-mouse CD25 (interleukin-2 receptor alpha chain) monoclonal antibody. It is used for flow cytometry and cell culture applications.

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JES6-5H4 (8 citations) Anti-IL-2 (clone JES6-5H4, (5 citations) IL-2 (JES6–5H4; (4 citations) Biotin anti-mouse IL-2 (clone JES6–5H4, (2 citations)

2 protocols using il 2 clone jes6 5h4

Immunophenotyping of Lung Infiltrating Cells

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Lung infiltrating immune cells were stained with different combinations of fluorochrome-coupled antibodies against mouse CD45 (clone 30-F11, Biolegend), CD3 (clone 17A2, Biolegend), CD4 (clone GK1.5, Biolegend), CD8 (clone 53-6.7, Biolegend), CD11b (clone M1/70, Biolegend), CD11c (clone N418, Biolegend), Foxp3 (clone FJK-16s, eBioscience), CD279 (PD-1, clone 29F.1A12, Biolegend), CD152 (CTLA-4, clone UC10-4B9, eBioscience), Tim-3 (clone RMT3–23, eBioscience), CD223 (Lag-3, clone C9B7W, Biolegend), IL-2 (clone JES6-5H4, Biolegend), IFNγ (clone XMG1.2, Biolegend), BrdU (clone Bu20a, Biolegend). Jurkat, PD-1-Jurkat, and human PBMCs were stained with fluorochrome-coupled antibodies against human CD3 (clone HIT3a, Biolegend), CD4 (clone OKT4, Biolegend), and CD279 (PD-1, clone EH12.2H7, Biolegend).

Deng J., Wang E.S., Jenkins R.W., Li S., Dries R., Yates K., Chhabra S., Huang W., Liu H., Aref A.R., Ivanova E., Paweletz C.P., Bowden M., Zhou C.W., Herter-Sprie G.S., Sorrentino J.A., Bisi J.E., Lizotte P.H., Merlino A.A., Quinn M.M., Bufe L.E., Yang A., Zhang Y., Zhang H., Gao P., Chen T., Cavanaugh M.E., Rode A.J., Haines E., Roberts P.J., Strum J.C., Richards W.G., Lorch J.H., Parangi S., Gunda V., Boland G.M., Bueno R., Palakurthi S., Freeman G.J., Ritz J., Haining W.N., Sharpless N.E., Arthanari H., Shapiro G.I., Barbie D.A., Gray N.S, & Wong K.K. (2017). CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation. Cancer discovery, 8(2), 216-233.

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Comprehensive Immunophenotyping by Flow Cytometry

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The following antibodies were used in an appropriate combination of fluorochromes: B220 (clone RA3–6B2, BioLegend), Bcl-6 (clone K112–91, BD Biosciences), CD4 (clone GK1.5, BioLegend), CD8α (clone 53–6.7, BioLegend), CD11a (clone M17/4, BioLegend), CD19 (clone 1D3/CD19, BioLegend), CD21 (Clone 7E9, BioLegend), CD23 (Clone B3B4, BioLegend), CD49d (clone R1–2, BioLegend), CD86 (Clone GL-1, BioLegend), CXCR5 (clone L138D7, BioLegend), FoxP3 (clone FJK-16s, eBioscience), GL7 (clone GL7, BioLegend), IFN-γ (clone XMG1.2, BioLegend), IgD (clone 11–26c.2a, BioLegend), IL-2 (clone JES6–5H4, BioLegend), IL-5 (clone TRFK5 BioLegend), IL-17A (clone TC11–18H10.1, BioLegend), PD-1 (clone 29F.1A12, BioLegend), T-bet (clone 4B10, BioLegend), TNF-α (clone MP6-XT22, BioLegend), and appropriate isotype controls.

Pardy R.D., Gentile M.E., Carter A.M., Condotta S.A., King I.L, & Richer M.J. (2022). An Epidemic Zika Virus Isolate Drives Enhanced T Follicular Helper Cell and B Cell-Mediated Immunity. Journal of immunology (Baltimore, Md. : 1950), 208(7), 1719-1728.

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