An Axio Observer D1 Microscope (Zeiss, Germany) equipped with an Alpha Plan-Apochromat 100 × /1.40 Oil immersion Objective (Zeiss, Germany) and an Evolve 512 × 512 EMCCD camera with pixel size 16.0 μm (Photometrics; Tucson, AZ) was used for in vitro condensate formation, immunofluorescence, and live-cell imaging. A Brightline® single-band laser filter set (Semrock; excitation filter: FF01–561/14, emission filter: FF01–609/54, and dichroic mirror: Di02-R561–25 × 36) was used for the excitation and emission of HaloTag® TMR ligand (Promega; G8251) or Alexa Fluor 568-labeled goat anti-rabbit (Life Technologies; A11011). A Brightline® single-band laser filter set (Semrock; excitation filter: FF02–482/18–25, emission filter: FF01–525/45–25, and dichroic mirror: Di02-R488–25 × 36) was used for the excitation and emission of Alexa Fluor 488-labeled goat anti-mouse (Life Technologies; A11029). The microscope and EMCCD camera were controlled by the computer via SlideBook 6.0 software (3i). Images were processed and presented using Adobe PhotoShop (Adobe Inc).
Brightline single band laser filter set
Manufactured by IDEX Corporation
The Brightline® single-band laser filter set is a precision optical filter designed to isolate a specific wavelength of light. It is intended for use in scientific and industrial applications that require the filtering of laser light.
Automatically generated - may contain errors
Lab products found in correlation
Halotag tmr ligand, by Promega (2 mentions)
Axio observer d1 microscope, by Zeiss (2 mentions)
Alexa fluor 488 labeled goat anti mouse, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 568 labeled goat anti rabbit, by Thermo Fisher Scientific (2 mentions)
Alpha plan apochromat 100 1.40 oil immersion objective, by Zeiss (2 mentions)
2 protocols using brightline single band laser filter set
1
Confocal Microscopy of Condensate Formation
2
Confocal Microscopy of Condensate Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An Axio Observer D1 Microscope (Zeiss, Germany) equipped with an Alpha Plan-Apochromat 100 × /1.40 Oil immersion Objective (Zeiss, Germany) and an Evolve 512 × 512 EMCCD camera with pixel size 16.0 μm (Photometrics; Tucson, AZ) was used for in vitro condensate formation, immunofluorescence, and live-cell imaging. A Brightline® single-band laser filter set (Semrock; excitation filter: FF01–561/14, emission filter: FF01–609/54, and dichroic mirror: Di02-R561–25 × 36) was used for the excitation and emission of HaloTag® TMR ligand (Promega; G8251) or Alexa Fluor 568-labeled goat anti-rabbit (Life Technologies; A11011). A Brightline® single-band laser filter set (Semrock; excitation filter: FF02–482/18–25, emission filter: FF01–525/45–25, and dichroic mirror: Di02-R488–25 × 36) was used for the excitation and emission of Alexa Fluor 488-labeled goat anti-mouse (Life Technologies; A11029). The microscope and EMCCD camera were controlled by the computer via SlideBook 6.0 software (3i). Images were processed and presented using Adobe PhotoShop (Adobe Inc).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!