Human cancer cell lines were cultured in RPMI-1640 (FUJIFILM Wako Pure Chemical Corporation) [PANC-1 (American Type Culture Collection), PK-1 (Cell Resource Center for Biomedical Research) and PCI-43 (provided by Dr Hiroshi Ishikura at Hokkaido University) (22 (link))] or high-glucose Dulbecco's modified Eagle's medium (DMEM; FUJIFILM Wako Pure Chemical Corporation) [SUIT-2 (Health Science Research Resources Bank) and MIA PaCa-II (JCRB Cell Bank)] supplemented with 10% (vol/vol) FBS (Biosera), 2 mM L-glutamine, 50 U/ml penicillin and 50 µg/ml streptomycin in a humidified atmosphere containing 5% CO2 at 37°C.
Suit 2
Manufactured by Health Science Research Resources Bank
Sourced in Japan
The SUIT-2 is a laboratory equipment designed for conducting various scientific experiments. It is a versatile and adaptable system that can be used for a wide range of applications. The core function of the SUIT-2 is to provide a controlled and monitored environment for research and testing purposes.
Automatically generated - may contain errors
Lab products found in correlation
Panc 1, by American Type Culture Collection (3 mentions)
Mia paca 2, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Miapaca2, by Health Science Research Resources Bank (2 mentions)
Aspc 1, by American Type Culture Collection (2 mentions)
Sw1990, by American Type Culture Collection (2 mentions)
Capan 2, by American Type Culture Collection (2 mentions)
Rpmi 1640, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Biosera (1 mentions)
Panc1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Suit 2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Gentlemacs dissociator, by Miltenyi Biotec (1 mentions)
Bxpc 3, by American Type Culture Collection (1 mentions)
Cfpac 1, by American Type Culture Collection (1 mentions)
Tumor dissociation kit, by Miltenyi Biotec (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Bronchial epithelial cell growth basal medium, by Lonza (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Apo transferrin, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
7 protocols using suit 2
1
Culturing Human Cancer Cell Lines
2
Human Pancreatic Cancer Cell Culture
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The human pancreatic cancer cell lines SUIT2 (Health Science Research Resources Bank), Panc-1 (American Tissue Culture Collection [ATCC]) and MIA-PaCa-II (JCRB Cell Bank) were grown in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) (SUIT2 and MIA-PaCa-II) or Roswell Park Memorial Institute (RPMI) 1640 (Panc-1) supplemented with 10% (vol/vol) fetal bovine serum (FBS), 2 mM l -glutamine, 50 U/ml penicillin and 50 µg/ml streptomycin in a humidified atmosphere containing 5% CO2. The cells were plated at a density of 105 cells/cm2.
3
Isolation and Characterization of Pancreatic Cell Types
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Human pancreatic cells were dissociated from surgical pancreatic tissues using a Tumor Dissociation Kit (human) and gentleMACS Dissociator (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to the manufacturer's instructions. Immediately after dissociation, the cells were analyzed by flow cytometry. In addition, we analyzed the following nine pancreatic cancer cell lines: BxPC3, CFPac-1, SW1990, AsPC1, and Capan-2 (American Type Culture Collection, Manassas, Va); Panc-1 (RIKEN, Tsukuba, Japan); MiaPaCa2, SUIT-2, and KP-2 (Health Science Research Resources Bank, Osaka, Japan). We also analyzed two normal pancreatic duct epithelial cell lines: a human primary normal pancreatic epithelial cell line, CS-PE (DS Pharma Biomedical Co., Osaka, Japan), and an immortalized pancreatic ductal epithelial cell line, HPDE6-E6E7 clone 6 (a gift from Dr. Ming-Sound Tsao, University of Toronto, Toronto, Canada). In addition, human pancreatic stellate cells (PSCs) were isolated from fresh pancreatic specimens using the outgrowth method [14] (link). The metastatic SUIT-2 cell line was previously established in our laboratory [15] (link). The same method was used to establish the metastatic Panc-1 cell line. Cells were maintained as described previously [16] (link).
4
Culturing Human Cancer and Normal Cells
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Human cancer cell lines were grown in McCoy’s 5A Medium (HCT116 [ATCC]), Roswell Park Memorial Institute (RPMI) 1640 (MKN45 [Japanese Cancer Research Resources Bank, Tsukuba, Japan], PANC-1 [ATCC], OE33 [DS Pharma Biomedical Co., Ltd., Osaka, Japan]) or high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (SUIT-2 [Health Science Research Resources Bank, Osaka, Japan]) supplemented with 10% (vol/vol) fetal bovine serum (FBS), 2 mM l -glutamine, 50 U/ml penicillin, and 50 µg/ml streptomycin in a humidified atmosphere containing 5% CO2. Human colorectal epithelial primary (CoEpiC; ScienCell Research Laboratories, Inc., CA, USA), Het1A (non-tumorous esophagus cells, ATCC) and HCEC-1CT (Summit Pharmaceuticals International Corporation, Tokyo, Japan) cells were grown in colonic epithelial cell medium (CoEpiCM; ScienCell), bronchial epithelial cell growth basal medium (Lonza, Basel, Switzerland) and ColoUp medium (DMEM/Medium 199 Earle’s, 4 + 1 (Biochrom Cat# F0435 and Cat# FG0615) containing 4 mM GlutaMAXTM-1 (100×), (Gibco, Cat# 35050-038) 2% cosmic calf serum (Hyclone, Cat# SH30087), 20 ng/ml EGF (Sigma Aldrich, Cat# E9644), 10 μg/ml Insulin (Sigma Aldrich, Cat# I9278), 2 μg/ml Apo-Transferrin (Sigma Aldrich, Cat# T2036), 5 nM sodium-selenite (Sigma Aldrich, Cat# S5261), and 1 μg/ml hydrocortisone (Sigma Aldrich, Cat# H0396)), respectively.
5
Isolation and Characterization of Human Pancreatic Stellate Cells
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Human PSCs were isolated from fresh pancreatic surgical specimens using the outgrowth method described by Bachem et al.27 , 28 The PSC cell type was confirmed by morphology (stellate‐like or spindle‐shaped cells) and by immunofluorescence staining for α‐smooth muscle actin (α‐SMA) and vimentin.28 , 29 , 30 The use of pancreatic cancer surgical specimens was approved by the Ethics Committee of Kyushu University (Fukuoka, Japan) and was carried out according to the Ethical Guidelines for Human Genome/Gene Research enacted by the Japanese Government and the Helsinki Declaration. We used 10 human PCC cell lines in this study: PANC‐1 (Institute of Physical and Chemical Research, Saitama, Japan), KP‐2 and KP‐3 (Japan Health Sciences Foundation, Tokyo, Japan), SUIT‐2 and MIA PaCa‐2 (Japan Health Science Research Resources Bank, Osaka, Japan), and AsPC‐1, CAPAN‐1, CAPAN‐2, Hs766T, and SW1990 (ATCC, Manassas, VA, USA), as well as normal human pancreatic epithelial cells (CS‐PE; Cell Systems, Applied Cell Biology Research Institute, Kirkland, WA, USA). The HPDE cell line was kindly provided by Dr. Ming‐Sound Tsao (University of Toronto, Toronto, ON, Canada). All of the cells were maintained as previously described.31
6
Isolation and Immortalization of Human Pancreatic Cancer Stem Cells
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We extracted human PSCs from fresh pancreatic cancer surgical specimens using the outgrowth method and immortalized them as described in our previous reports (20 (link),21 (link)). The pancreatic cancer cell line SUIT-2 was purchased from Japan Health Science Research Resources Bank (Osaka, Japan). Both PSCs and SUIT-2 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum at 37°C and 10% CO2, as described previously (22 (link)). Cells were confirmed to be free of mycoplasma and used within eight passages for each experiment.
7
Culturing Human Cancer and Epithelial Cells
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Human CRC cells (HCT116 and SW480) (American Type Culture Collection [ATCC]), human pancreatic cancer cells (SUIT‐2) (Health Science Research Resources Bank), and human colon epithelial cells (HCEC‐1CT) (Summit Pharmaceuticals International Corporation) were cultured in the appropriate medium10 in a humidified atmosphere with 5% CO2 at 37°C.
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