Sheep kidney cells (SKCs) were isolated from Chinese Merino fetuses (∼45 d) that were derived from a livestock slaughterhouse in Shihezi City (China) and cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% non-essential amino acids (NEAA; Gibco), penicillin-streptomycin (100×, Coolaber, China), and 100 μg/ml Primocin (Invitrogen). ICR mouse embryonic fibroblasts (MEFs; Cyagen, China) were treated with mitomycin C (Sigma) as feeder layers and cultured in SKC medium without Primocin. Sheep iPSCs were cultured on inactivated ICR MEFs in DMEM/F12 medium (Gibco) supplemented with 20% KnockOut Serum Replacement (Gibco), 1% NEAA, 1% GlutaMAX (Gibco), 0.1 mM β-mercaptoethanol (Sigma), 4 ng/ml basic fibroblast growth factor (bFGF; Peprotech), 1 μg/ml doxycycline hyclate (DOX; Sigma), 50 μg/ml vitamin C (Vc; Sigma), and 1 mM valproic acid (VPA; Sigma). Embryoid body (EB) formation medium (Cyagen, China) was composed of 440 ml basal medium, 50 ml fetal bovine serum, 5 ml NEAA, 500 μl 2-mercaptoethanol, 5 ml L-glutamine, and 5 ml penicillin-streptomycin.
Penicillin streptomycin
Manufactured by Coolaber
Sourced in China, Israel
Penicillin-streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics used to prevent bacterial contamination in cell culture media.
Automatically generated - may contain errors
Lab products found in correlation
Mitomycin c, by Merck Group (1 mentions)
Doxycycline hyclate dox, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions)
Primocin, by Thermo Fisher Scientific (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Cyagen (1 mentions)
Primocin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Dmem medium, by Sartorius (1 mentions)
Hepes buffer, by Procell (1 mentions)
2 protocols using penicillin streptomycin
1
Sheep iPSC Culture Protocol
2
Culturing SW480 and SW1116 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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SW480 and SW1116 cells were acquired from ATCC and grown in DMEM medium (Biological Industries, Israel) supplemented with 10% FBS (Biological Industries, Israel), 1% penicillin/streptomycin (Coolaber, Beijing, China), and 2.5% HEPES buffer (Procell, Wuhan, China) in an incubator with a humidified air atmosphere of 5% CO2 at 37°C.
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