CSF was collected and analyzed for α-syn, total tau (t-tau), tau phosphorylated at Thr181 (p-tau), and Aβ42 as previously described (Pagano et al., 2016 (link), 2018 (link)). Briefly, CSF was collected using standardized lumbar puncture procedures. CSF was collected into siliconized polypropylene tubes, and the first 1 to 2 mL of CSF was sent to the site’s local laboratory for routine testing for cell count, total protein level, and glucose level. An additional 15 to 20 mL of CSF was transferred into15-mL conical polypropylene tubes at room temperature, mixed gently, centrifuged at 2000 g for 10 min at room temperature, and transferred into 1.5-mL precooled siliconized polypropylene aliquot tubes followed by immediate freezing on dry ice. The frozen aliquots of CSF were shipped overnight to the PPMI Biorepository Core laboratories on dry ice and then thawed, aliquoted into 0.5-mL siliconized polypropylene tubes, refrozen once, and stored at −80°C. Measurements of CSF Ab42, tau, and p-tau were made using the xMAP-Luminex platform with INNOBIA AlzBio3 immunoassay kit–based reagents (Fujirebio-Innogenetics, Ghent, Belgium) at Penn. Commercially available sandwich type immunoassay kits (BioLegend; formerly Covance) were used to analyze CSF a-syn and CSF hemoglobin levels.
Further information can be found in the PPMI biologics manual (see text footnote 1).
Further information can be found in the PPMI biologics manual (see text footnote 1).