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2 protocols using s 2 4 isothiocyanatobenzyl diethylenetriamine pentaacetic acid p scn bn dtpa

1

Radiolabeling of Albumin-based Nanoparticles

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Human serum albumins and glutaraldehyde solution (25% v/v) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Anhydrous ethanol (>95%) was purchased from J.T. Baker Inc. (Phillipsburg, NJ, USA). Hydrogen tetrachloroaurate (III) hydrate (HAuCl4ยท3H2O) was purchased from Alfa Aesar (Haverhill, MA, USA). Sodium citrate dehydrate was purchased from Merck (Darmstadt, Germany). S-2-(4-Isothiocyanatobenzyl)-diethylenetriamine pentaacetic acid (p-SCN-Bn-DTPA) was purchased from Macrocyclics (Dallas, TX, USA). The 111In-InCl3 solution was obtained from the Institute of Nuclear Energy Research (Taoyuan, Taiwan). Cell culture dishes, flasks, and plastic ware were purchased from Corning Inc (Corning, NY, USA). Fetal bovine serum and cell culture medium were purchased from HyClone (Logan, UT, USA). Sepharose 4B gel and Poly-Prep chromatography columns were purchased from GE Healthcare (Chalfont St. Giles, UK) and Bio-Rad (Hercules, CA, USA), respectively.
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2

Radiolabeling Bovine Serum Albumin

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Bovine serum albumins (laboratory grade albumin, albumin tissue culture grade and low endotoxin, essentially IgG free albumin) were purchased from Sigma (Dorset, UK). S-2-(4-Isothiocyanatobenzyl)-diethylenetriamine pentaacetic acid (p-SCN-Bn-DTPA) was purchased from Macrocyclics (Dallas, USA). 111InCl3 was purchased from Mallinckrodt Medical Inc. (Petten, Netherlands). Phosphate buffered saline (PBS) tablets were purchased from Oxoid (Basingstoke, UK) and prepared using ultrapure water according to the manufacturer's instructions. Foetal bovine serum was purchased from Life Technologies (USA). Diethylenetriamine pentaacetic acid (DTPA) and ammonium acetate were purchased from Fluka (UK). Sodium hydroxide (NaOH) and Tris(hydroxymethyl)aminomethane (Tris) were purchased from Fisher Scientific (Loughborough, UK). Glutaraldehyde (50% vol/vol in water) was purchased from Aldrich (UK). Ethanol was purchased from VWR (Radnor, USA).
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