Alexa fluor 488 conjugated cholera toxin β ctb

Manufactured by Thermo Fisher Scientific

Alexa Fluor 488-conjugated cholera toxin-β (CTB) is a fluorescently labeled protein used for biological research applications. CTB is the non-toxic, binding subunit of cholera toxin and is commonly used as a marker for retrograde tracing and visualization of neuronal pathways. The Alexa Fluor 488 dye provides a green fluorescent signal that can be detected using standard fluorescence microscopy techniques.

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Lab products found in correlation

Tetramethylindocarbocyanine perchlorate dii, by Merck Group (1 mentions)

Close spelling variants of this product

Alexa Fluor 488‐conjugated CTB Alexa 488-conjugated CTB CTB-Alexa-488 Cholera toxin Alexa Fluor 488 CTB-488 Alexa 488 Alexa Fluors

2 protocols using alexa fluor 488 conjugated cholera toxin β ctb

Vaginal Innervation Tracing in Mice

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All surgical procedures were performed under aseptic conditions in a designated animal surgery area. Mice were anesthetized by inhaled isoflurane (4% induction, 2.5% maintenance), and using a Hamilton microsyringe (33-gauge needle), multiple 1.5-2 μL injections of Alexa Fluor 488-conjugated cholera toxin-β (CTB, 2 mg/mL in saline, 8 μL total; Invitrogen, Grand Island, NY) were made circumferentially within the distal region of the vagina, approximately 3-5 mm from the external opening. Using a separate Hamilton microsyringe (33-gauge needle), 2-3 μL injections of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; 2% in DMSO, 5 μL total; Sigma) were made on the right and left side, subcutaneously, at the junction between the hairy skin and the opening of the vagina. Mice were returned to their home cages and allowed to recover from anesthesia under observation.

Pierce A.N., Zhang Z., Fuentes I.M., Wang R., Ryals J.M, & Christianson J.A. (2015). Neonatal vaginal irritation results in long-term visceral and somatic hypersensitivity and increased hypothalamic–pituitary–adrenal axis output in female mice. Pain, 156(10), 2021-2031.

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Vaginal Tract Labeling in Mice

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All surgical procedures were performed under aseptic conditions in a designated animal surgery area. Mice were anesthetized by inhaled isoflurane (4% induction, 2.5 % maintenance) and using a Hamilton microsyringe (33 gauge needle), multiple 1.5-2µl injections of Alexa Fluor 488-conjugated cholera toxin-β (CTB, 2mg/ml in saline, 8µl total; Invitrogen) were made circumferentially within the distal region of the vagina, approximately 3–5mm from the external opening. Using a separate Hamilton microsyringe (33 gauge needle), 2–3µl injections of 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; 2% in DMSO, 5µl total; Sigma, St. Louis, MO) were made on the right and left side, subcutaneously, at the junction between the hairy skin and the opening of the vagina. Mice were returned to their home cages and allowed to recover from anesthesia under observation.

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