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Pgd2 mox elisa

Manufactured by Cayman Chemical
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The PGD2-MOX ELISA is a quantitative enzyme-linked immunosorbent assay (ELISA) used for the measurement of 15-Deoxy-Δ⁠12,14-Prostaglandin J₂ (15d-PGJ₂) in biological samples. It is designed to detect and quantify the levels of this metabolite of Prostaglandin D₂ (PGD₂) in various matrices.

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Lab products found in correlation

V plex proinflammatory panel 1 human kit, by Mesoscale (1 mentions) Mouse il 13 elisa, by Thermo Fisher Scientific (1 mentions) Pge2 elisa, by R&D Systems (1 mentions) Mouse xl cytokine array kit, by R&D Systems (1 mentions) Pierce bca protein assay, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PGD2: MOX ELISA Kit  PGD2-MOX Express ELISA kit PGD2-MOX ELISAs

3 protocols using pgd2 mox elisa

1

Cytokine Profiling via ELISA

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Human IL-13 ELISA (cn: 435207, Biolegend), mouse IL-13 ELISA (cn: 88-7137-22, eBioscience), human IL-33 (cn: 435907, Biolegend), human IL-25 ELISA (cn: KA2190, Abnova), human TSLP ELISA (cn: 434207, Biolegend), PGD2-MOX ELISA (cn: 512011, Cayman Chemicals) were performed according to the manufacturer’s instructions. Human IL-4 and IL-17 were quantified using V-Plex Proinflammatory Panel 1 (human) Kit (cn: K15049D, Meso Scale Discovery), according to the manufacturer’s instruction.
Trabanelli S., Chevalier M.F., Martinez-Usatorre A., Gomez-Cadena A., Salomé B., Lecciso M., Salvestrini V., Verdeil G., Racle J., Papayannidis C., Morita H., Pizzitola I., Grandclément C., Bohner P., Bruni E., Girotra M., Pallavi R., Falvo P., Leibundgut E.O., Baerlocher G.M., Carlo-Stella C., Taurino D., Santoro A., Spinelli O., Rambaldi A., Giarin E., Basso G., Tresoldi C., Ciceri F., Gfeller D., Akdis C.A., Mazzarella L., Minucci S., Pelicci P.G., Marcenaro E., McKenzie A.N., Vanhecke D., Coukos G., Mavilio D., Curti A., Derré L, & Jandus C. (2017). Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis. Nature Communications, 8, 593.
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2

Cytokine and Prostaglandin Profiling

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CM was collected from each group after centrifugation at 700 × g for 10 min at 4°C and stored at −80°C for subsequent experiments. For cytokine measurement, conditioned medium was subjected to a proteome profiler array using a mouse XL cytokine array kit (R&D, ARY028), and the relative levels of 111 cytokines and chemokines were measured. The array procedure and data analysis were performed according to the manufacturer’s instructions.
For measurement of prostaglandins, CM were collected as described and analyzed according to the manufacturer’s instructions. The ELISA kit used for measurement were as follows: PGD2-MOX ELISA(Cayman Chemical company, 500151); PGJ2 ELISA(ENZO, ADI-900-023); PGE2ELISA(R&D systems, KGE004B).
Lemaire V., Koehl M., Le Moal M, & Abrous D.N. (2000). Prenatal stress produces learning deficits associated with an inhibition of neurogenesis in the hippocampus. Proceedings of the National Academy of Sciences of the United States of America, 97(20), 11032-11037.
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3

PGD2 Quantification by ELISA

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PGD2-MOX ELISA (CaymanChemicals, Ann Arbor, Michigan, USA) was performed according to the manufacturer’s protocol. The standards and the samples were diluted in a 1:1 mixture of culture medium and methyl-oximating reagent and assayed at adequate dilutions and in duplicates, where possible. In some experiments, obtained values were normalized to the total protein content per well as determined with a PierceTM BCA protein assay (ThermoFisher Scientific) or tissue wet weight (PCLS).
Rittchen S., Jandl K., Lanz I., Reiter B., Ferreirós N., Kratz D., Lindenmann J., Brcic L., Bärnthaler T., Atallah R., Olschewski H., Sturm E.M, & Heinemann A. (2021). Monocytes and Macrophages Serve as Potent Prostaglandin D2 Sources during Acute, Non-Allergic Pulmonary Inflammation. International Journal of Molecular Sciences, 22(21), 11697.
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