Interleukin 1β il 1β kit

EMO was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). LF was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China), and HA was provided by Bloomage Biotechnology Co., Ltd. (Shandong, China). CS and β-GP were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO) was purchased from J&K Scientific (Beijing, China). Coumarin-6 (C₆) was purchased from Aladdin Reagent Company (Shanghai, China), and an antifade mounting medium with DAPI was obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). The tumor necrosis factor-alpha (TNF-α) kit, interleukin-1β (IL-1β) kit, and interleukin-6 (IL-6) kit were purchased from MultiScience (Lianke) Biotech Co., Ltd. (Hangzhou, China). The malondialdehyde (MDA) kit was purchased from ElabScience Biotech Co., Ltd. (Wuhan, China). Anti-myeloid differentiation factor 88 (MyD88) and anti-TLR4 antibodies were supplied by Servicebio Technology Co., Ltd. (Wuhan, China), and anti-NF-κB p65 was provided by ImmunoWay Biotechnology Company (St. Plano, TX, USA). All chemicals used in the study were not further purified.

Dextran sulfate sodium (DSS, 36–50 kDa) was purchased from Seebio^® Biomedicals (Xian, China). Shellac (purity ≥ 95%, batch no. BCI-16072505) was procured from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai, China). The zein protein (purity ≥ 98%) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Curcumin (purity ≥ 98%) and IR780 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol was procured from Aladdin (Shanghai, China). The myeloperoxidase (MPO) kit, interleukin-6 (IL-6) kit, interleukin-1β (IL-1β) kit, and tumor necrosis factor-alpha (TNF-α) kit, were supplied by MultiScience (Lianke) Biotech Co., Ltd. (Zhejiang, China).
Male BALB/c mice (22–25 g) were obtained from SPF Biotechnology Co., Ltd. (Beijing, China). The mice were kept under standard conditions and freely supplied with food and distilled water. All animal experiments were performed in accordance with the protocol approved by the Animal Ethics Committee of Chengdu University of Traditional Chinese Medicine.

CPT-11 (Irinotecan HCl Trihydrate, M_w 677.19) was purchased from Melonepharma Co., Ltd. (Dalian, China). Weikeqi Biological Technology Co., Ltd (Chengdu, China) supplied the reference compounds Puerarin, Coptisine, Berberine, Palmatine, Daidzin, Liquiritin, Daidzein, Wogonoside, Wogonin, Baicalin and Baicalein (purity ≥ 98%). HPLC-grade reagent were obtained from Thermo Fisher Scientific (Thermo scientific, USA). The tumor Necrosis factor-α (TNF-α) kit, interleukin 1β (IL-1β) kit, and interleukin 6 (IL-6) kit were purchased from Multi Science (Lianke) Biotech Co., Ltd. (Hangzhou, China). PI3K antibody (60225-1-Ig), NF-κB p65 antibody (66535-1-Ig) were purchased from Proteintech Group, Inc (Wuhan, China). Abmart Shanghai Co., Ltd (Shanghai, China) provided the AKT (T55561) antibody and Servicebio Biotechnology Co., Ltd. (Wuhan, China) provided the β-actin antibody (GB12001). Professor Guihua Jiang (College of Pharmacy, Chengdu University of Traditional Chinese Medicine, China) identified all of the herbs procured from the Sichuan Neautus Traditional Chinese Medicine Co., Ltd. (Chengdu, China).