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Cd34 fitc 8g12

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The CD34 FITC (8G12) is a fluorescein isothiocyanate (FITC) conjugated monoclonal antibody that binds to the CD34 antigen. CD34 is a glycoprotein expressed on the surface of hematopoietic stem and progenitor cells.

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Lab products found in correlation

Cd235a pe gpa ga r2, by BD (2 mentions) Hcd45 v450 hi30, by BD (2 mentions) Cd71 pe cy7 okt9, by Thermo Fisher Scientific (2 mentions) Cd49d bv421 9f10, by BD (2 mentions)

Close spelling variants of this product

Anti human CD34 FITC (8G12, (2 citations) FITC) anti-CD34 (clone 8G12; (2 citations) CD34-FITC (clone 8G12; (4 citations) CD34-FITC (134 citations)

2 protocols using cd34 fitc 8g12

1

Erythrocyte Differentiation of Edited HSPCs

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HSPC were differentiated as described above and then were harvested at day 14-16 for analyses of erythrocyte lineage specific markers. Cells were spun down and washed and then erythrocyte differentiation of edited (WT and HiFi Cas9) and non-edited HSPCs was assessed by flow cytometry using the following antibodies: hCD45 V450 (HI30, BD Biosciences), CD34 FITC (8G12, BD Biosciences), CD71 PE-Cy7 (OKT9, Affymetrix), and CD235a PE (GPA) (GA-R2, BD Biosciences). Reticulocyte differentiation was analyzed using the following antibodies: CD235a PE (GPA) (GA-R2, BD Biosciences), CD49d BV421 (9F10, BD Biosciences), and Band3 APC (a kind gift from Dr. Anumpama Narla and Dr. Mohan Narla).
Vakulskas C.A., Dever D.P., Rettig G.R., Turk R., Jacobi A.M., Collingwood M.A., Bode N.M., McNeill M.S., Yan S., Camarena J., Lee C.M., Park S.H., Wiebking V., Bak R.O., Gomez-Ospina N., Pavel-Dinu M., Sun W., Bao G., Porteus M.H, & Behlke M.A. (2018). A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human haematopoietic stem and progenitor cells. Nature medicine, 24(8), 1216-1224.
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2

Erythrocyte Differentiation of Edited HSPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
HSPC were differentiated as described above and then were harvested at day 14-16 for analyses of erythrocyte lineage specific markers. Cells were spun down and washed and then erythrocyte differentiation of edited (WT and HiFi Cas9) and non-edited HSPCs was assessed by flow cytometry using the following antibodies: hCD45 V450 (HI30, BD Biosciences), CD34 FITC (8G12, BD Biosciences), CD71 PE-Cy7 (OKT9, Affymetrix), and CD235a PE (GPA) (GA-R2, BD Biosciences). Reticulocyte differentiation was analyzed using the following antibodies: CD235a PE (GPA) (GA-R2, BD Biosciences), CD49d BV421 (9F10, BD Biosciences), and Band3 APC (a kind gift from Dr. Anumpama Narla and Dr. Mohan Narla).
Vakulskas C.A., Dever D.P., Rettig G.R., Turk R., Jacobi A.M., Collingwood M.A., Bode N.M., McNeill M.S., Yan S., Camarena J., Lee C.M., Park S.H., Wiebking V., Bak R.O., Gomez-Ospina N., Pavel-Dinu M., Sun W., Bao G., Porteus M.H, & Behlke M.A. (2018). A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human haematopoietic stem and progenitor cells. Nature medicine, 24(8), 1216-1224.
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