Sodium fluorescein naf

Manufactured by Merck Group

Sourced in United States

Sodium fluorescein (NaF) is a water-soluble dye commonly used in various laboratory applications. It is a fluorescent compound that absorbs light in the blue-green region of the visible spectrum and emits light in the yellow-green region. The core function of sodium fluorescein is to serve as a tracer or marker in various analytical and research techniques, where its fluorescent properties are utilized to visualize and track specific substances or processes.

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Lab products found in correlation

Dulbecco phosphate buffered saline (dpbs), by Merck Group (1 mentions) Millicell ers 2, by Merck Group (1 mentions) Occludin, by Abcam (1 mentions) N acetylcysteine amide naca, by Merck Group (1 mentions) Trpm2, by Abcam (1 mentions) Jam a, by Abcam (1 mentions) β actin, by Signalway Antibody (1 mentions)

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Sodium fluorescein (145 citations)

3 protocols using sodium fluorescein naf

Evaluating BBB Barrier Function via TEER

Cited 1 time

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BBB barrier function was evaluated by measuring TEER. The resistance values (Ω) were measured using a Millicell ERS-2 (Merk Millipore). The TEER values were calculated as previously described [10 (link), 11 (link)]. We confirmed the value of TEER was peaked on day 10. Therefore, all experiments were performed on day 10.
The cells were washed with Dulbecco’s phosphate-buffered saline (DPBS, Sigma) supplemented with D-glucose and HEPES (DPBS-H). DPBS-H containing 10 μg/mL sodium fluorescein (NaF, Sigma) as a paracellular marker was then added into the upper chamber for 30 min at 37 °C. After incubation, the medium in the lower chamber was collected and then the concentration of NaF in the medium was measured using a fluorescence multi-well plate reader (Genios, TECAN).

Zhang H., Yamaguchi T, & Kawabata K. (2023). The maturation of iPS cell-derived brain microvascular endothelial cells by inducible-SOX18 expression. Fluids and Barriers of the CNS, 20, 10.

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HIV-1 Tat-Induced Barrier Dysfunction

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METH was purchased from the National Institutes for Food and Drug Control (Cat#: 171212-200603, Beijing, China). Recombinant HIV-1 Tat Clade-B was purchased from Prospec (Tat, Cat#: HIV-129, Rehovot, Israel). N-acetylcysteine amide (NACA, Cat#: A0737), 8-Bromo-cyclic adenosine diphosphate ribose (8-Br-cADPR, Cat#: B5416), Evans Blue (EB, Cat#: E2129), and sodium fluorescein (NaF, Cat#: F6377) were purchased from Sigma-Aldrich (Missouri, United States). DeadEnd™ Fluorometric TUNEL System was purchased from Progema (Cat#: G3250, Fitchburg, United States). The antibodies used were JAMA (Cat#: ab180821, 1:1,000, Abcam, United Kingdom), Occludin (Cat#: ab167161, 1:5,000, Abcam, United Kingdom), ZO1 (Cat#: ab216880, 1:1,000, Abcam, United Kingdom), TRPM2 (Cat#: ab11168, 1:1,000, Abcam, United Kingdom), β-Actin (Cat#: 21,338, 1:1,000, Signalway Antibody, United States), and secondary antibody (Cat#: L3012, 1:5,000, Signalway Antibody, United States).

Huang J., Zhang R., Wang S., Zhang D., Leung C.K., Yang G., Li Y., Liu L., Xu Y., Lin S., Wang C., Zeng X, & Li J. (2021). Methamphetamine and HIV-Tat Protein Synergistically Induce Oxidative Stress and Blood-Brain Barrier Damage via Transient Receptor Potential Melastatin 2 Channel. Frontiers in Pharmacology, 12, 619436.

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Endothelial Barrier Permeability Assay

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The permeability of endothelial monolayer was determined using Sodium Fluorescein (Na‐F) (100 ng/mL; Sigma‐Aldrich Cat# F6377). The transcytosis system was constructed by confluence hCMEC/D3 cells on inserts with collagen‐coated polycarbonate transwell membrane filters (cell culture inserts; Millicell Cell Culture Inserts, RRID:SCR_015799). The hCMEC/D3 monolayer was cultured in the apical side of a 24‐well tissue culture insert. The EBM‐2 medium was added into both the apical side and the basolateral side. After P. gingivalis infection at the apical side for 12 hr, all EBM‐2 medium was replaced by warmed Assay Buffer. Fluorescence labeled Na‐F was added into the apical Assay Buffer, after 5 min, inserts were removed and the fluorescence of the basolateral Assay Buffer was determined by a reader (infiniteM200 TECAN, excitation wavelength, 485 nm; measurement wavelength, 535 nm). These experiments were carried out at 37°C.

Zeng F., Liu Y., Huang W., Qing H., Kadowaki T., Kashiwazaki H., Ni J, & Wu Z. (2020). Receptor for advanced glycation end products up‐regulation in cerebral endothelial cells mediates cerebrovascular‐related amyloid β accumulation after Porphyromonas gingivalis infection. Journal of Neurochemistry, 158(3), 724-736.

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