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Fastrna blue

Manufactured by MP Biomedicals
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FastRNA Blue is a reagent designed for the efficient extraction and purification of total RNA from a variety of biological samples. It utilizes a guanidinium-based lysis solution and a silica-based membrane technology to isolate high-quality RNA.

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Lab products found in correlation

Sybr greener kit, by Thermo Fisher Scientific (2 mentions) Abi prism 7500, by Thermo Fisher Scientific (2 mentions) Superscript first strand synthesis system for rt pcr, by Thermo Fisher Scientific (2 mentions) Ribopure rna purification kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FastRNA Pro Blue Kit FastRNA Blue Kit

2 protocols using fastrna blue

1

Bacterial gene expression analysis by qPCR

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Bacterial RNA was isolated using FastRNA Blue (MP Biomedicals) according to manufacturer´s guidelines. cDNA synthesis was performed using the Superscript first-strand synthesis system for RT-PCR (Invitrogen). The real-time PCR amplification was performed with SYBR GreenER Kit (Invitrogen) using an ABI Prism 7500 sequence detection system (Applied Biosystems). The level of gyrA transcription was used for normalization of bacterial genes. The primers used in this study are published by Watanabe et al.33 (link).
Siemens N., Kittang B.R., Chakrakodi B., Oppegaard O., Johansson L., Bruun T., Mylvaganam H., Svensson M., Skrede S, & Norrby-Teglund A. (2015). Increased cytotoxicity and streptolysin O activity in group G streptococcal strains causing invasive tissue infections. Scientific Reports, 5, 16945.
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2

RNA Isolation and qRT-PCR Analysis

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Bacterial RNA was isolated using FastRNA Blue (MP Biomedicals) and eukaryotic RNA was isolated using RiboPure RNA purification Kit (Ambion) according to manufacturer´s guidelines. cDNA synthesis was performed using the Superscript first-strand synthesis system for RT-PCR (Invitrogen). Primer sets used are summarized in Supplementary Table 3. The real-time PCR amplification was performed with SYBR GreenER Kit (Invitrogen) using an ABI Prism 7500 sequence detection system (Applied Biosystems). The levels of gyrA or β-actin transcription were used for normalization.
Mairpady Shambat S., Siemens N., Monk I.R., Mohan D.B., Mukundan S., Krishnan K.C., Prabhakara S., Snäll J., Kearns A., Vandenesch F., Svensson M., Kotb M., Gopal B., Arakere G, & Norrby-Teglund A. (2016). A point mutation in AgrC determines cytotoxic or colonizing properties associated with phenotypic variants of ST22 MRSA strains. Scientific Reports, 6, 31360.
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