Anti toll like receptor 4 antibody anti tlr4

The fruiting bodies of the wild mushroom A. hemibapha subspecies javanica were purchased from the local market in Chiang Mai, Thailand, in 2018. They were carefully washed with tap water, and dried using a hot air oven at 60 °C for 3 h. The dried sample was finely milled with a grinder into powder, and stored at −20 °C. RPMI-1640 cell culture medium, fetal bovine serum (FBS), streptomycin, and penicillin were purchased from Gibco Life Technologies (Grand Island, NY, USA). Griess reagent was purchased from Sigma-Aldrich (NSW, Australia). The EZ-Cytox cell viability assay kit (WST-1) was purchased from Daeil Lab Service Co., Ltd., Korea. The anti-complement receptor 3 antibody (anti-CR3), and anti-toll-like receptor 4 antibody (anti-TLR4) were obtained from Abcam (Cambridge, MA, USA). The phospho-NF-κB antibody, phospho-p38 antibody (MAPK), phospho-ERK (MAPK) antibody, and phospho-JNK antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals, and reagents used in this study were of high analytical grade.

The media and other materials required for culturing cells were purchased from Lonza Inc. (Walkersville, MD, USA). Griess reagent and lipopolysaccharide (LPS: E. coli, serotype O111:B4; L2630) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The WST-1 assay kit was obtained from Daeillab Service Co., Korea. Anti-Toll-like receptor 2 antibody (anti-TLR2), anti-complement receptor 3 antibody (anti-CR3), and anti-Toll-like receptor 4 antibody (anti-TLR4), were obtained from Abcam (Cambridge, MA, USA). Phospho-NF-κB antibody, phospho-p38 (MAPK) antibody, phospho-ERK (MAPK) antibody, and phospho-JNK antibody were purchased from Cell Signalling Technology (Danvers, MA, USA). All chemicals and reagents used in this work were of analytical grade.