Mouse α cyclin b2

Manufactured by Santa Cruz Biotechnology

Mouse α-cyclin B2 is a protein that plays a crucial role in cell cycle regulation. It functions as a regulatory subunit of the cyclin-dependent kinase CDC2, which is essential for the transition from G2 phase to mitosis.

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Lab products found in correlation

Mouse α cdc27, by BD (2 mentions) Rabbit α ppp1a pt320, by Abcam (2 mentions) Rabbit α cdc25c, by Abcam (2 mentions) Rabbit α ensa ps67 arpp19 ps62, by Cell Signaling Technology (2 mentions) Rabbit α cdk1 py15, by Cell Signaling Technology (2 mentions) Amdex goat α rabbit igg, by GE Healthcare (2 mentions) Criterion tris hcl precast gel, by Bio-Rad (2 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (2 mentions) Supersignal west maximum sensitivity substrate, by Thermo Fisher Scientific (2 mentions) Sheep α mouse igg, by GE Healthcare (2 mentions)

Close spelling variants of this product

Cyclin B2 (3 citations)

2 protocols using mouse α cyclin b2

Western Blot Analysis of Cell Cycle Regulators

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein samples were resolved on 10% Criterion Tris-HCl precast gels (Biorad, #3450011) or in the case of the phosphorylated species of Arpp19 and ENSA on a 10% Tris-HCl SDS-PAGE supplemented with 10 μM Phos-tag reagent (NARD Institute, AAL-107) and then transferred onto PVDF membranes. The following antibodies were used for detection of the respective proteins: mouse α-Cdc27 (BD Biosciences, #610455), mouse α-cyclin B2 (Santa Cruz Biotechnology, #sc-53239), rabbit α-Nup53 serum,^{85 (link)} rabbit α-PPP1A pT320 (Abcam, #ab62334), rabbit α-Cdc25C,^{44 (link)} rabbit α-Wee1 pT150,^{86 (link)} rabbit α-Greatwall serum,^{60 (link)} rabbit α-ENSA serum,^{60 (link)} rabbit α-Arpp19,^{63 (link)} rabbit α-ENSA pS67/Arpp19 pS62 (Cell Signaling Technology, #5240) and rabbit α-Cdk1 pY15 (Cell Signaling Technology, #9111L). For the detection of the primary antibody, peroxidase-linked sheep α-mouse IgG (GE Healthcare, #NA931V) and AMDEX goat α-rabbit IgG (GE Healthcare, RPN4301) was used. Chemiluminescence was detected on a BioRad ChemiDoc MP Imaging system using SuperSignal West Maximum Sensitivity Substrate (ThermoFisher Scientific, #34095) or Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore, #WBLKS0500).

Kamenz J., Gelens L, & Ferrell JE J.r. (2020). Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate Phosphorylation. Current biology : CB, 31(4), 794-808.e6.

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Western Blot Analysis of Cell Cycle Regulators

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kamenz J., Gelens L, & Ferrell JE J.r. (2020). Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate Phosphorylation. Current biology : CB, 31(4), 794-808.e6.

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