Hdac glo 2 assay

Manufactured by Promega

Sourced in United States

The HDAC-Glo 2 Assay is a luminescent-based assay designed to measure the activity of histone deacetylases (HDACs) in cell-based and biochemical samples. It provides a simple, sensitive, and high-throughput method for detecting HDAC activity.

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Lab products found in correlation

S30 t7 high yield protein expression system, by Promega (1 mentions) Glomax luminometer, by Promega (1 mentions) Safire2 plate reader, by Tecan (1 mentions)

3 protocols using hdac glo 2 assay

Luminescent HDAC Activity Assay

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The HDAC-Glo 2 Assay and HDAC-Glo class IIa Assay (Promega, Madison, WI, USA) were used to determine lysate activities. Luminescence was measured using a Panomics Luminometer (Gentaur, Kampenhout, Belgium).

Wang S.E., Ko S.Y., Kim Y.S., Jo S., Lee S.H., Jung S.J, & Son H. (2018). Capsaicin upregulates HDAC2 via TRPV1 and impairs neuronal maturation in mice. Experimental & Molecular Medicine, 50(3), e455-.

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HDAC2 Activity Assay Using NLuc

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HDAC2-NLuc, HDAC2 and NLuc were expressed in the S30 T7 High-Yield Protein expression system (Promega) as recommended by the manufacturer. A total of 40 μl of each expression reaction (in triplicates) were tested for HDAC2 activity using HDAC-Glo 2 assay (Promega). Luminescence was measured on a GloMAX luminometer (Promega).

Robers M.B., Dart M.L., Woodroofe C.C., Zimprich C.A., Kirkland T.A., Machleidt T., Kupcho K.R., Levin S., Hartnett J.R., Zimmerman K., Niles A.L., Ohana R.F., Daniels D.L., Slater M., Wood M.G., Cong M., Cheng Y.Q, & Wood K.V. (2015). Target engagement and drug residence time can be observed in living cells with BRET. Nature Communications, 6, 10091.

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HDAC2 Activity Quantification Assay

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HDAC2 enzyme activity was assessed in cells treated for 24 h with 50 nM entinostat or mock using the HDAC-Glo 2 Assay (Promega), nonlytic format, and quantified on a Safire² plate reader (Tecan). Raw data were corrected for background luminescence and normalized to mock to determine relative HDAC2 activity.

Vendetti F.P., Topper M., Huang P., Dobromilskaya I., Easwaran H., Wrangle J., Baylin S.B., Poirier J.T, & Rudin C.M. (2014). Evaluation of azacitidine and entinostat as sensitization agents to cytotoxic chemotherapy in preclinical models of non-small cell lung cancer. Oncotarget, 6(1), 56-70.

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