Anti igd apc

Manufactured by Thermo Fisher Scientific

Sourced in Germany

The Anti-IgD-APC is a laboratory reagent used for the detection and identification of IgD-positive cells in flow cytometry analysis. It contains an anti-IgD antibody conjugated to the fluorescent dye allophycocyanin (APC).

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Lab products found in correlation

Igm pe, by Thermo Fisher Scientific (1 mentions) Anti cd19 pecy7, by Thermo Fisher Scientific (1 mentions) Anti cd20 apc, by Thermo Fisher Scientific (1 mentions) Igd ia6 2, by BioLegend (1 mentions) Anti igm apc, by Thermo Fisher Scientific (1 mentions) Evos fl digital inverted fluorescence microscope, by Thermo Fisher Scientific (1 mentions) Anti cd90.1 pe, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-IgD

2 protocols using anti igd apc

FACS and PLA Analysis of Mouse and Human B Cells

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For FACS analysis of mouse spleen B cells or transfected TKO cells, following fluorophore- conjugated anti-mouse antibodies were used: Anti-CD45R-PerCP-Cy5.5 (RA3-6B2), Anti-IgM-APC, IgM-PE, IgM-FITC (II/41; all eBioscience, Frankfurt, Germany), Anti-CD20-APC, Anti-CD19-PE-Cy7, anti-IgD-AF647, Anti-IgD-APC, Anti-IgD-PE (11-26c.2a; all eBioscience).
For PLA probes against specific targets, the following unlabelled antibodies were used: IgD (11-26c.2a, SouthernBiotech, Birmingham, AL), IgD (AMS9.1; Santa Cruz Biotechnology, Dallas, TX), IgM (R33.24.12, in house hybridoma culture), IgM (rabbit anti-mouse µHC; Rockland Immunochemicals, Gilbertsville, PA), IgM (1B4B1; SouthernBiotech), Lambda light chain (JC5-1; SouthernBiotech), Kappa light chain (187.1; SouthernBiotech), CD19 (6D5; AbD Serotec, Düsseldorf, Germany) and CD20 (AISB12; eBioscience). Igα (HMK7/A9; abcam, Cambridge, UK), Syk (Syk-01; BioLegend, San Diego, CA).
For PLA probes against human BCR, the following unlabelled antibodies were used: IgD (IA6-2; BioLegend), IgD (IADB6) and IgM (SA-DA4) from Acris Antibodies (Herford, Germany), and IgM (Fc5u) from Genway Biotech (San Diego, CA).

Kläsener K., Maity P.C., Hobeika E., Yang J, & Reth M. (2014). B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk. eLife, 3, e02069.

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Visualizing T Cell Localization in Spleen GCs

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To visualize the location of CD90.1⁺ OT-II cells relative to GCs in B6, Sle2c2 or BATF3^−/− mice, 7-mm-thick OCT-embedded spleen cryosections were prepared on Superfrost slides, washed with PBS, fixed with 4% paraformaldehyde for 15 min at 4°C, and subsequently permeabilized with 0.1% Triton X-100 for 5 min at 4°C. The sections were washed with cold PBS and incubated overnight in the dark at 4°C with anti-CD90.1-PE (Clone OX-7, Biolegend), anti-T- and B-Cell Activation Antigen conjugated to FITC (Clone GL7, BD Biosciences) and anti-IgD-APC (Clone 11–26c, eBioscience). Stained sections were washed three times with cold PBS, mounted with Cytoseal, and covered with a glass coverslip. Images of stained sections were captured with an EVOS FL digital inverted fluorescence microscope (Fisher Scientific) keeping all microscope conditions and software settings identical for all treatments and controls.

Abboud G., Elshikha A.S., Kanda N., Zeumer-Spataro L, & Morel L. (2022). Contribution of dendritic cell subsets to T cell-dependent responses in mice. Journal of immunology (Baltimore, Md. : 1950), 208(5), 1066-1075.

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