Bead homogenizer

Manufactured by Omni International

The Bead Homogenizer is a laboratory equipment used for the disruption and homogenization of samples. It utilizes high-speed agitation of sample material with beads to efficiently break down and mix solid, semi-solid, or liquid samples.

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Lab products found in correlation

Sybr green, by Bio-Rad (1 mentions) Complete edta free protease inhibitor cocktail, by Merck Group (1 mentions)

Spelling variants of this product

Bead Mill Homogenizer (26 citations) Omni Bead Ruptor Homogenizer (16 citations) Bead Ruptor Elite Bead Mill Homogenizer (16 citations) Bead Ruptor 12 homogenizer (5 citations) Omni Bead Ruptor 24 Bead Mill Homogenizer (3 citations) Bead Ruptor 24 Elite Homogenizer (3 citations) Bead Ruptor 4 Homogenizer (2 citations) Bead Ruptor 24 Elite bead mill homogenizer (2 citations) Bead homogenizer device (2 citations) Omni Bead Ruptor 12 Homogenizer (2 citations)

2 protocols using bead homogenizer

Quantitative RT-PCR from Frozen Tissue

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Aliquots of pulverized frozen tissue were placed in homogenization
tubes on ice with 5–6 ceramic beads. 1 mL cold TRIzol lysis reagent
(ThermoFisher 15596026) was added to each sample. Samples were then
homogenized in a bead homogenizer (Omni International) for two cycles of 20
seconds separated by a 10-second pause. Total RNA was then isolated
following manufacturers instructions. Samples were then reverse transcribed
using Applied Biosystems High-Capacity cDNA Reverse Transcription Kit
according to instructions (ThermoFisher 4368813). Quantitative real-time PCR
was performed on the cDNAs in the presence of fluorescent dye (SYBR green,
BioRad) using primers listed in Supplemental Table 6.
Expression levels were determined using the comparative cycle threshold
(2^−ΔΔCt) method.

Murashige D., Jung J.W., Neinast M.D., Levin M.G., Chu Q., Lambert J.P., Garbincius J.F., Kim B., Hoshino A., Marti-Pamies I., McDaid K., Shewale S.V., Flam E., Yang S., Roberts E., Li L., Morley M.P., Bedi KC J.r., Hyman M.C., Frankel D.S., Margulies K.B., Assoian R.K., Elrod J.W., Jang C., Rabinowitz J.D, & Arany Z. (2022). Extra-cardiac BCAA catabolism lowers blood pressure and protects from heart failure. Cell metabolism, 34(11), 1749-1764.e7.

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Preparation and Preservation of Mouse Serum, Cell Lysates, and Tumor Lysates

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Mouse blood was collected through terminal cardiac puncture and spun at 2,000 × g for 5 min. The resulting serum layer was collected and stored at −80°C until use. Cell lysate preparation for cGAMP degradation activity assay is as following: cells from a confluent well in a 6 well plate was collected in 1 mL of PBS, centrifuged at 1000 × g for 3 minutes, lysed in 50–100 μL of lysis buffer (10 mM Tris pH 9, 150 mM NaCl, 10 μM ZnCl₂, 1% NP-40), and stored in −20°C until use. For western blotting, cells were lysed on the plate in 100–250 μL of Laemmli sample buffer, boiled at 95°C for 5 minutes and sonicated. Mouse tumor lysate (100 mg/mL) for cGAMP degradation activity assay were generated by lysing tissues in 10 mM Tris pH 7.5, 150 mM NaCl, 10 μM ZnCl₂, 1.5% NP-40, and freshly added protease inhibitors (cOmplete, EDTA-free protease inhibitor cocktail, Sigma-Aldrich). Organ lysates were then homogenized with a bead homogenizer (Omni International) and stored at −20°C until use.

Wang S., Böhnert V., Joseph A.J., Sudaryo V., Swinderman J., Yu F.B., Lyu X., Skariah G., Subramanyam V., Gilbert L.A., Goodarzi H, & Lingyin L. (2023). ENPP1 is an innate immune checkpoint of the anticancer cGAMP-STING pathway. bioRxiv.

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