Icycler myiq 2 two color real time detection system

Gene expression analysis of for spätzle, relish, toll, myD88, eater, and vg was performed by iCycler MyiQ™2 Two-color Real-Time Detection System (Bio-Rad™) in Hard-Shell High-Profile Semi-skirted 96-Well PCR Plates with rp49 as a housekeeping gene. Reaction mixtures contained 10 µl of SsoAdvanced™ Universal SYBR^® Green Supermix, 0.5 µM of forward and reverse primers (final concentration), and 300 ng of cDNA, and ultra-pure H₂O was added to a total volume of 20 µl.
Reaction cycles set: 1× (30 s at 95°C); 45× (10 s at 95°C, 30 s at 58°C, 30 s at 72°C) followed by melt curve analysis increasing temperature from 55 to 95°C. Each sample was analyzed with two technical replicates to check for repeatability.

Gene expression analysis of for spätzle, relish, toll, myD88, eater, and vg was performed by iCycler MyiQ™2 Two-color Real-Time Detection System (Bio-Rad™) in Hard-Shell High-Profile Semi-skirted 96-Well PCR Plates with rp49 as a housekeeping gene. Reaction mixtures contained 10 µl of SsoAdvanced™ Universal SYBR^® Green Supermix, 0.5 µM of forward and reverse primers (final concentration), and 300 ng of cDNA, and ultra-pure H₂O was added to a total volume of 20 µl.
Reaction cycles set: 1× (30 s at 95°C); 45× (10 s at 95°C, 30 s at 58°C, 30 s at 72°C) followed by melt curve analysis increasing temperature from 55 to 95°C. Each sample was analyzed with two technical replicates to check for repeatability.