Cell apoptosis was detected using the flow cytometric Annexin V-FITC/PI double staining with the commercialized Annexin-VFITC/PI Apoptosis Detection Kit (BD Biosciences, USA) on a FACSAria™ Fusion Flow Cytometers (BD Biosciences, USA), following the manufacturer’s instruction. Briefly, all treated cell lines were first seeded in the 96-well plate and incubated overnight. After that, samples were incubated with FITC-Annexin V (5 μL) and propidium iodide (0.1 μg) for 15 min at room temperature, respectively. Finally, apoptotic rates were profiled through the flow cytometer.
Facs aria fusion flow cytometers
Manufactured by BD
Sourced in United States
The BD FACS Aria Fusion flow cytometers are designed for high-performance cell analysis and sorting. These instruments use laser-based technology to rapidly analyze and sort individual cells within a sample, providing detailed information about cellular characteristics and populations.
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Lab products found in correlation
Annexin 5 fitc pi apoptosis detection kit, by BD (1 mentions)
Transcription buffer set, by BD (1 mentions)
Cd16 32 fc block, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Carboxyfluorescein succinimidyl ester (cfse), by Merck Group (1 mentions)
Anti b220 microbeads, by Miltenyi Biotec (1 mentions)
Foxp3 transcription buffer set, by Thermo Fisher Scientific (1 mentions)
3 protocols using facs aria fusion flow cytometers
1
Apoptosis Quantification by Flow Cytometry
2
Intracellular Flow Cytometry of Zbtb46
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Cells were kept at 4 °C while being stained in PBS with 0.5% BSA and 2 mM EDTA in the presence of CD16/32 Fc block (BD; clone 2.4G2). For intracellular flow cytometry, cells were stained for surface markers, permeabilized, and fixed with the transcription buffer set (BD) following the manufacturer’s instructions. Cells were then stained for intracellular Zbtb46 expression at 4 °C on ice. Cells were analyzed on a FACS Canto II and sorted on a FACS Aria Fusion flow cytometers (BD). Data were analyzed with FlowJo software (Tree Star). Antibodies and other staining materials are described in SI Appendix, Materials and Methods .
3
Comprehensive Immune Cell Profiling
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