To study integrin regulation of MMP expression, primary mouse anti-human integrin β1 (clone P4C10), integrin β2 (clone MEM48), integrin β3 (clone B3A), FITC-conjugated anti-human integrin β1, integrin αV (clone 272-17E6) antibodies were used (all from Millipore, Hertfordshire, UK). FITC conjugated goat anti-Mouse IgG1 (Sigma-Aldrich, Dorset, UK), and Cy5 conjugated goat anti-rabbit (Abcam, Cambridge, UK) were used as secondary antibodies. Mouse IgG1 and FITC-conjugated Mouse IgG1 were the isotype controls (BD Diagnostics, Oxford, UK).
Fitc conjugated goat anti mouse igg1
Manufactured by Merck Group
Sourced in United Kingdom
FITC conjugated goat anti-mouse IgG1 is a secondary antibody used in immunoassays and other research applications. It is produced by immunizing goats with mouse IgG1 and conjugating the resulting antibodies with the fluorescent label FITC (fluorescein isothiocyanate).
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Integrin regulation of MMP expression
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Integrin Regulation of MMP Expression
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To study integrin regulation of MMP expression, primary mouse anti-human integrin β1 (clone P4C10), integrin β2 (clone MEM48), integrin β3 (clone B3A), FITC-conjugated anti-human integrin β1, and integrin αV (clone 272-17E6) Abs were used (all from Millipore, Hertfordshire, U.K.). FITC-conjugated goat anti-mouse IgG1 (Sigma-Aldrich, Dorset, U.K.), and Cy5 conjugated goat anti-rabbit (Abcam, Cambridge, U.K.) were used as secondary Abs. Mouse IgG1 and FITC-conjugated mouse IgG1 were the isotype controls (BD Diagnostics, Oxford, U.K.).
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