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5 protocols using propan 2 ol

1

Synthesis of dye-doped ZnO nanoparticles

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Titanium(IV) isopropoxide (TTIP, 97%), C.I. Basic Blue 9 (Methylene Blue—MB, 95%), C.I. Basic Red 1 (Rhodamine 6G—R6G, 95%) and C.I. Basic Violet 10 (Rhodamine B—RhB, 95%) were purchased from Sigma-Aldrich. Zinc acetate dihydrate (99.5%), propan-2-ol (IPA, 99.5%), and ammonia (25%) were purchased from Chempur (Piekary, Śląskie, Poland). All reagents were of analytical grade and used without any further purification. The water used in all experiments was deionized.
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2

Lipase-Catalyzed Synthesis of POSS-based Biohybrid Materials

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α-Chitin from crab shells, lipase type B from Candida antarctica (CALB), 4-nitrophenyl palmitate (p-NPP), gum arabic and Triton X-100 were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Methacryl POSS® cage mixture (MPOSS), Aminoethylaminopropylisobutyl POSS® (APOSS), Epoxycyclohexylisobutyl POSS® (EPOSS), and Vinyl POSS® cage mixture (VPOSS) were purchased from Hybrid Plastics (Hattiesburg, MS, USA). Propan-2-ol and NaOH was purchased from Chempur (Gliwice, Poland), and 10 mM phosphate buffer at pH 7 was obtained from Amresco Company (Solon, OH, USA). Commercially available rapeseed oil was bought from a local factory.
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3

Solvent Characterization for Ibuprofen Extraction

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All reagents were commercially available materials and were used without further purification. (RS)-Ibuprofen (99%) was obtained from Acros Organics (Geel, Belgium). l-Valine (≥99%) was purchased from Carl Roth (Karlsruhe, Germany). Trimethylsilyl chloride (≥99%) (TMSCl) was provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Methanol (MeOH), ethanol (EtOH), propan-2-ol (iPrOH), propan-1-ol (PrOH), butan-1-ol (BuOH), pentan-1-ol (AmOH), hexan-1-ol (HexOH), heptan-1-ol (HeptOH), acetic acid, potassium chloride, sodium chloride, orthophosphoric acid (98%), diethyl ether was high purity obtained from Chempur (Gliwice, Poland). Ammonium hydroxide solution 25% (NH3·H2O) was of analytical grade purchased from StanLab (Lublin, Poland). Acetonitrile (≥99.9%) for HPLC gradient grade and n-octanol (≥99%) were provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Disodium hydrogen phosphate dihydrate (≥99%) (Na2HPO4·2H2O), disodium hydrogen phosphate dodecahydrate (99%) (Na2HPO4·12H2O) were provided by Fisher Bioreagents (Pittsburgh, Pennsylvania, USA) and sodium dihydrogen phosphate anhydrous (98%) (NaH2PO4) was obtained from Acros Organics (Geel, Belgium). Potassium dihydrogen phosphate anhydrous (99%) was obtained from Merck (Darmstadt, Germany). Deuterated chloroform (CDCl3) (99.8%) (+0.03% TMSCl) was purchased from Eurisotop (Cheshire, England).
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4

Optimized Purification of Ibuprofen

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All reagents were commercially available materials and were used without further purification. (RS)-Ibuprofen (99%) was obtained from Acros Organics (Geel, Belgium). L-valine and L-leucine (≥99%) were purchased from Carl Roth (Karlsruhe, Germany). Trimethylsilyl chloride (≥99%) (TMSCl) was provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Methanol, propan-2-ol (iPrOH), potassium chloride, sodium chloride, orthophosphoric acid (98%), pH = 4 buffer, citric acid, sodium hydroxide were high purity obtained from Chempur (Piekary Ślaskie, Poland). Ammonium hydroxide solution 25% (NH3∙H2O) was of analytical grade purchased from StanLab (Lublin, Poland). Acetonitrile (≥99.9%) for HPLC gradient grade was provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Anhydrous disodium hydrogen phosphate (≥99%) (Na2HPO4), anhydrous potassium dihydrogen phosphate (99%) (KH2PO4) were provided by Merck (Darmstadt, Germany). Yeast extract and bacto-peptone were obtained from Graso BIOTECH (Starogard Gdański, Poland). D-mannitol (≥99.9%) was provided by POL-AURA (Dywity, Poland).
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5

Synthesis of Photocatalytic Nanocomposites

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Titanium(IV) isopropoxide (TTIP, 97%), zirconium(IV) propoxide (TZIP, solution 70 wt.% in 1-propanol) and tetracycline (TC, 98%) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Zinc acetate dihydrate (ZA, 99.5%) was purchased from Merck KGaA (Darmstadt, Germany). Ammonia (25%) and propan-2-ol (IPA, 99.5%) were purchased from Chempur (Piekary Śląskie, Poland). Deionized water was used in all experiments. All reagents were used without any further purification.
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