Concentration of lipid hydroperoxides were determined using a modified method of Yi et al [15] . Sample (0.3 mL) was mixed with 1.5 mL isooctane/2-propanol (3:2, v:v), vortex-mixed three times, and centrifuged for 3 min at 2000 g. The upper layer of 0.2 mL was collected and mixed with 2.8 mL methanol/1-butanol (2:1, v:v). Thiocyanate/Fe2+ solution (30 μL) was added to the mixture and the mixture was vortex-mixed for 10 s. The samples were incubated for 30 min at room temperature and the absorbance at 510 nm was measured using an UV/VIS-spectrometer (Model UV-1650PC; Shimadzu, Kyoto, Japan). The concentration of lipid hydroperoxide was calculated using a cumene hydroperoxide standard curve.
Model uv 1650pc
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu Model UV-1650PC is a UV-Vis spectrophotometer designed for laboratory applications. It measures the absorbance or transmittance of light in the ultraviolet and visible wavelength regions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using model uv 1650pc
1
Quantifying Lipid Hydroperoxide Levels
2
Light Transmittance and Color Analysis of PLA Composites
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Light transmittance curves of PLA and PLA/nanocelluloses composites were obtained in the range of 200–800 nm using a Shimadzu UV-visible spectrophotometer (Model UV-1650pc). The color of the films was evaluated using a Minolta colorimeter (CR-20, Konica Minolta, Inc., Tokyo, Japan) previously calibrated with a white reflector plate. Measurements were expressed as colorimetric coordinates in the CIELAB scale: L* (lightness, from 0: black to 100: white), a* (from green (−) to red (+)), and b* (from blue (−) to yellow (+)). Color differences (ΔE) were calculated using Equation (2):
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