Vaccine or IgG ELISAs were performed with a 1:20 dilution of the 2014-2015 seasonal influenza vaccine, 8HAU inactivated H1N1 A/California/07/09 virus or 2ug/uL of anti-IgG antibody per well. Antibodies were serially diluted threefold seven times with starting concentrations of 10 ug/mL. Microtiter plates were coated with diluted vaccine or anti-IgG antibody. The well- characterized Cr9114 antibody was used as a positive control for the vaccine ELISAs, recombinant human IgG was used for the IgG ELISAs. Intermediate washes were performed with PBS/0.05% Tween. A horseradish peroxidase-conjugated goat anti-human IgG antibody (Jackson ImmunoResearch Laboratories) was used as a secondary and plates were developed with SuperAquaBlue (EBioscience) until the positive controls reached an OD405 of 3.0.
Super aquablue
Manufactured by Thermo Fisher Scientific
Sourced in Sweden, United States
The Super AquaBlue is a laboratory equipment designed for precise measurement of water-related parameters. It features advanced sensor technology to accurately monitor factors such as pH, conductivity, and dissolved oxygen levels. The Super AquaBlue is a versatile tool suitable for various water testing applications in research and industrial settings.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase conjugated goat anti human igg antibody, by Jackson ImmunoResearch (1 mentions)
Milli q system, by Merck Group (1 mentions)
Cnbr activated sepharose 4b, by GE Healthcare (1 mentions)
Goat anti mouse immunoglobulin g igg, by Jackson ImmunoResearch (1 mentions)
Cnbr activated sepharose, by GE Healthcare (1 mentions)
Mc lr standard, by Enzo Life Sciences (1 mentions)
3 protocols using super aquablue
1
Influenza Vaccine and IgG ELISA Protocol
2
Quantification of Zearalenone using ELISA
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Zearalenone (ZEN) was purchased from Fermentek (Jerusalem, Israel). Mouse monoclonal antibodies against ZEN (anti-ZEN) and ZEN conjugated with horseradish peroxidase (ZEN-HRP) were produced at Ghent University (Ghent, Belgium) according to Burmistrova et al (2009) [33] . Goat anti-mouse immunoglobulin G (IgG) was purchased from Jackson ImmunoResearch Laboratories Inc (Suffolk, UK). CNBr activated Sepharose 4B was purchased from GE Healthcare (Uppsala, Sweden). Super AquaBlue, the ABTS-based ELISA substrate, a product of eBioscience was from AH diagnostics AB (Solna, Sweden). All chemicals were of analytical grade.
Carrier buffer (100 mM phosphate buffer containing 50 mM NaCl, pH 7.4) and regeneration buffer (200 mM glycine-HCl, pH 2.4) were prepared in ultrapure water from the Milli-Q system from Millipore (Bredford, MA, USA) and filtered using an analytical filter paper from Munktell (Grycksbo, Sweden). All aqueous solutions were also prepared with the same ultrapure water. All the chemicals, if not otherwise specified, were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Carrier buffer (100 mM phosphate buffer containing 50 mM NaCl, pH 7.4) and regeneration buffer (200 mM glycine-HCl, pH 2.4) were prepared in ultrapure water from the Milli-Q system from Millipore (Bredford, MA, USA) and filtered using an analytical filter paper from Munktell (Grycksbo, Sweden). All aqueous solutions were also prepared with the same ultrapure water. All the chemicals, if not otherwise specified, were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Microcystin-LR Detection Protocol
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CNBr-activated Sepharose was purchased from GE Healthcare (Uppsala, Sweden). Super AquaBlue was purchased from eBioscience (San Diego, CA, USA), MCLR standard was from Enzo Life Sciences (Malmö, Sweden), Adda-specific monoclonal antibodies were from Abraxis LLC (Warminster, PA, USA). Float-A-lyzer G2 dialysis membrane (MWCO, 20 kDa) and HRP were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). DS 110 screen-printed electrodes (SPEs) were from DropSens (Llanera, Spain). All other chemicals used in this work were of analytical grade. Buffers were prepared using deionized water purified with Millipore (18.2 MΩ cm resistivity) (Millipore, Bedford, MA, USA).
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