Mln4924 b1036

Manufactured by Apexbio

Sourced in United States

MLN4924 (B1036) is a small molecule compound that inhibits the NEDD8-activating enzyme (NAE). NAE is an essential enzyme responsible for the activation of the ubiquitin-like protein NEDD8, which plays a crucial role in the regulation of cullin-RING E3 ubiquitin ligases. By inhibiting NAE, MLN4924 disrupts the NEDD8 conjugation pathway, leading to the stabilization of cullin-RING E3 ubiquitin ligase substrates and the induction of cellular stress responses.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Skbr3, by American Type Culture Collection (1 mentions) H1299, by American Type Culture Collection (1 mentions) Hek293, by American Type Culture Collection (1 mentions) Cisplatin, by Selleck Chemicals (1 mentions) Pd150606 d5946, by Merck Group (1 mentions)

2 protocols using mln4924 b1036

Cell Culture and Compound Treatment

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HEK293, H1299, SK-BR3, MDA-MB-231, and MCF7 cells were obtained from American Type Culture Collection (ATCC) and were authenticated by ATCC. Cells were expanded and preserved in liquid nitrogen upon receipt. Cells for experiments were passaged for fewer than 25-30 times. All cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (11995; Invitrogen), supplemented with 10% (v/v) of fetal bovine serum (10091148; Invitrogen) and 1% (v/v) of penicillin-streptomycin (15140-163; Invitrogen) at 37°C in a humidified incubator with 5% of CO 2 . MLN4924 (B1036) and CHX (C7698) were obtained from Apexbio and Sigma, respectively.

Xiong X., Cui D., Bi Y., Sun Y, & Zhao Y. (2020). Neddylation modification of ribosomal protein RPS27L or RPS27 by MDM2 or NEDP1 regulates cancer cell survival. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(10).

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Drug Resistance Mechanism Research

Cited 1 time

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All cell lines were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Drug resistant cells SGC-R and BGC-R cells were developed from SGC7901 and BGC823, respectively 16 (link). All cell lines were cultured as previously reported. The chemical reagents including Dichloroacetic acid (DCA) (D54702), KNK437 (SML0964), Cycloheximide (R750107), chloroquine (C6628), MG132 (474790) and PD150606 (D5946) were purchased from Sigma Aldrich (USA). MLN4924 (B1036) and radicicol (A4067) were purchased from ApexBio (USA). Cisplatin and KRIBB11 (S840201) were obtained from Selleck (Shanghai, China).

Xu J., Shi Q., Xu W., Zhou Q., Shi R., Ma Y., Chen D., Zhu L., Feng L., Cheng A.S., Morrison H., Wang X, & Jin H. (2019). Metabolic enzyme PDK3 forms a positive feedback loop with transcription factor HSF1 to drive chemoresistance. Theranostics, 9(10), 2999-3013.

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