Infectious progeny assays were conducted as follows. HeLa cells seeded in 24-well plates were infected with CTL2 as described. Infected cells were lysed at 48 h p.i. by adding Nonidet P40 (NP40) at a final concentration of 0.06% per well. Pipetting steps were performed using a robotic station (Biomek FX pipetting robot, Beckman Coulter). Lysates were diluted in infection medium and transferred to infect fresh HeLa cells at a final dilution of 1:40. Plates were incubated for 24 h at 35 °C and 5% CO2 and processed for immunolabeling.
Biomek fx pipetting robot
Manufactured by Beckman Coulter
The Biomek FX pipetting robot is a laboratory automation device designed for precise and efficient liquid handling tasks. It features a robotic arm that can be programmed to perform a variety of pipetting operations, including sample transfers, reagent addition, and serial dilutions. The Biomek FX offers high-precision liquid handling capabilities to support a wide range of applications in life science research and drug discovery.
Automatically generated - may contain errors
Lab products found in correlation
Biomek software, by Beckman Coulter (1 mentions)
Datacontrol 384, by Nanion Technologies (1 mentions)
Patchmaster, by HEKA Elektronik (1 mentions)
Patchliner octo, by Nanion Technologies (1 mentions)
Syncropatch 384pe, by Nanion Technologies (1 mentions)
Patchcontrol 384, by Nanion Technologies (1 mentions)
Igor pro, by Wavemetrics (1 mentions)
2 protocols using biomek fx pipetting robot
1
Quantifying Viral Progeny Production
2
Automated High-throughput Patch Clamp Recordings
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SyncroPatch 384PE (Nanion Technologies) described here is an automated patch clamp module in combination with a 384-channel digital amplifier (Tecella), a Biomek FX pipetting robot (Beckman Coulter) with 384 pipettor, software for control of the robot (Biomek Software, Beckman Coulter), and proprietary software for data acquisition (PatchControl 384, Nanion Technologies) and data analysis (DataControl 384, Nanion Technologies). Data output and compound information is compatible with most database formats. For the patch clamp recordings, planar borosilicate glass patch clamp chips were used 18 in a 384-microtiter-plate format. For Patchliner experiments, a Patchliner Octo (Nanion Technologies) was used, including HEKA EPC10 Quadro amplifiers (HEKA Elektronik, Lambrecht, Germany). PatchControlHT (Nanion Technologies), the software for controlling the experiment, was integrated with PatchMaster (HEKA Elektronik) for acquisition of data and online analysis of the recorded data. Data were exported and analyzed offline using IGOR Pro (WaveMetrics, Lake Oswego, OR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!