Phospho erk thr202 tyr204 4370

Manufactured by Cell Signaling Technology

Sourced in Germany

Phospho-ERK (Thr202/Tyr204) (#4370) is a primary antibody that recognizes endogenous levels of ERK1 and ERK2 only when dually phosphorylated at Thr202 and Tyr204.

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Lab products found in correlation

Aperio scanscope system, by Leica (1 mentions)

Close spelling variants of this product

Phospho-ERK (527 citations) ERK/phospho-ERK (3 citations) Phospho-ERK #4370 (2 citations)

2 protocols using phospho erk thr202 tyr204 4370

Immunohistochemical Analysis of p65BTK

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Specimens from patients admitted to Desio Hospital (n=13) were fixed with formalin, dehydrated, diaphanized with xylene, put in paraffin and processed for tissue microarray. Slides were stained according to standard immunohistochemistry procedures with the following primary antibodies: anti-hnRNPK (sc-25373) from Santa Cruz Biotechnologies (Heidelberg, Germany); phospho-ERK (Thr202/Tyr204) (#4370) from Cell Signaling (Danvers, MA, USA); and anti-p65BTK BN49 polyclonal antibody. Slides were digitally acquired using Aperio ScanScope System (Leica Microsystems, Milano, Italy). On specimens from patients admitted to Trieste University Hospital (n=83), p65BTK staining was graded accordingly to an increasing intensity by blind reading by two experienced operators and classified as negative, positive and strongly positive.

Grassilli E., Pisano F., Cialdella A., Bonomo S., Missaglia C., Cerrito M.G., Masiero L., Ianzano L., Giordano F., Cicirelli V., Narloch R., D'Amato F., Noli B., Ferri G.L., Leone B.E., Stanta G., Bonin S., Helin K., Giovannoni R, & Lavitrano M. (2016). A novel oncogenic BTK isoform is overexpressed in colon cancers and required for RAS-mediated transformation. Oncogene, 35(33), 4368-4378.

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Immunoblotting of MAPK Signaling

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Immunoblotting (IB) was conducted as in previous studies.¹⁵ Antibodies specific for SENP3 (5591), JNK (9252), phospho‐SPAK/JNK (Thr183/Tyr185) (4668), p38 (8690), phospho‐p38 (Thr180/Tyr182) (4511), p44/42 MAPK (Erk1/2) (4695) and phospho‐ERK (Thr202/Tyr204) (4370) were all purchased from Cell Signaling Technology. Anti‐murine TF antibody was purchased from R&D Systems (AF3178).

Chen X., Lao Y., Yi J., Yang J., He S, & Chen Y. (2020). SENP3 in monocytes/macrophages up‐regulates tissue factor and mediates lipopolysaccharide‐induced acute lung injury by enhancing JNK phosphorylation. Journal of Cellular and Molecular Medicine, 24(10), 5454-5462.

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