μbondapak

An Agilent 1200 series HPLC (Stockport, Cheshire, UK) was used to analyse the drug content of the powders, filaments and films. To quantify ibuprofen, a C18 column was used with a mobile phase consisting of: 0.5 volumes of phosphoric acid, 340 volumes of acetonitrile and 600 volumes of water then equilibrate and dilute to 1000 volumes with water. Weighed amount of ibuprofen standard and ibuprofen containing samples were dissolved in 2 mL of acetonitrile separately, and sonicated for 10 minutes. The resulting solution was diluted to 10 mL by using the mobile phase. The flow rate was set at 2 mL/min, and the detection spectrophotometer was set at 214 nm with injection volume of 20 µL.
Weighed amounts of the paracetamol standard and samples were transferred into volumetric flask containing mobile phase (3:1, methanol: water), sonicated for 2 minutes. Flow rate was set at 1.5 mL/min; detection spectrophotometer was set at 243 nm, and sample volume was 10 µL. The stationary phase comprised of a C-18 column, μbondapak (300 mm×3.9 mm; Waters®, USA). The content uniformity was conducted only for formulation E (PVA films containing paracetamol). A calibration curve was prepared for paracetamol with a linear relationship between 0.017-1.56 mg/mL (R 2 = 0.9976). Three films were analysed.