Nutrient broth nb

No-carbon essential (NCE) ^{31 (link)} with glycerol (22 mM) as the carbon and energy source was used as minimal growth medium. When added to the medium, the following supplements were at the indicated concentrations: trace minerals ^{32 (link)} (10 mL L⁻¹), MgSO₄ (1 mM), 5,6-dimethylbenzimidazole (DMB, 0.15 mM), ampicillin (0.1 mg mL⁻¹), arabinose (0.5 mM). All corrinoids (cobyric acid dicyanide [(CN)₂Cby], cobinamide dicyanide [(CN)₂Cbi], and cyanocobalamin (CNCbl)) were added at (1 or 10 nM) final concentrations. When ethanolamine (90 mM) was used as a carbon and energy source, Fe(III)-citrate (0.05 mM) was also added to the medium with corrinoids (300 nM). (CN)₂Cby was a gift from Paul Renz (Universität-Hohenheim, Stuttgart, Germany). All other chemicals were purchased from Sigma-Aldrich. S. enterica strains were cultured in Nutrient Broth (NB, Difco Laboratories) (0.8% w/v) containing NaCl (85 mM). Lysogeny broth (LB) ^{33 (link)}, ^{34 (link)} was used as rich medium to culture Escherichia coli strains unless otherwise indicated.

No-carbon essential (NCE)^{65 (link)} medium supplemented with glycerol (22 mM) as the carbon and energy source was used as a minimal growth medium. When added to the medium, the following supplements were at the indicated concentrations: trace minerals (10 mL L⁻¹)^{66 (link)}, MgSO₄ (1 mM), 5,6-dimethylbenzimidazole (DMB, 0.15 mM), ampicillin (0.1 mg mL⁻¹), arabinose (0.25 mM). Cobyric acid dicyanide [(CN)₂Cby] and cyanocobalamin (CNCbl) were added at (1 nM) final concentrations. (CN)₂Cby was a gift from Paul Renz (Universität-Hohenheim, Germany). All other chemicals were purchased from Sigma-Aldrich. S. enterica strains were cultured in Nutrient Broth (NB, Difco Laboratories)⁶⁷ containing NaCl (85 mM). Lysogenic broth (LB)^{68 (link), 69 (link)} was used as a rich medium to culture Escherichia coli strains.