Hydrogenated soybean phosphatidylcholine hspc

1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000) and hydrogenated soybean phosphatidylcholine (HSPC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Doxorubicin hydrochloride (DOX·HCl) was supplied by Hisun Pharmaceutical (Zhejiang, China). Cholesterol was obtained from Hushi Chemical (Shanghai, China). ICG was supplied by TCI (Tokyo, Japan). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT reagent) and Hoechst 33,324 were purchased from Sigma-Aldrich (St Louis, MO, USA). Triethylamine (TEA), NaN₃, and 4-dimethylaminopyridine (DMAP) were obtained from Sinopharm Chemical Reagent (Shanghai, China). Live/Dead viability/cytotoxicity kit was purchased from Invitrogen (Carlsbad, CA, USA). 1,3-Diphenylisobenzofuran (DPBF) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were obtained from Beyotime Institute of Biotechnology (Jiangsu, China). The chemicals and solvents were of analytical grade and used as received without further purification.