Human liver cancer line HepG2 (ATCC HB-8065) and human breast cancer line MCF-7 (ATCC HTB-22) and normal human liver cell line HL-7702 were purchased from ATCC company. HepG2 cells were cultured in growth medium (WME supplemented with 5% fatal bovine serum (FBS), 10 mM Hepes, 2 mM l -glutamine, 5 µg/mL insulin, 0.05 µg/mL hydrocortisone, 50 units/mL penicillin, 50 µg/mL streptomycin, and 100 µg/mL gentamycin). MCF-7 cells and HL-7702 cells were cultured in DMEM supplemented with 10% (v/v) FBS and 50 units/mL of penicillin and 50 µg/mL of streptomycin and 100 µg/mL gentamycin. HepG2 and MCF-7 cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2.
Hl 7702
Manufactured by American Type Culture Collection
Sourced in United States
The HL-7702 is a cell line derived from human liver tissue. It is commonly used in in vitro research applications to study liver-related biological processes and disease models.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by American Type Culture Collection (50 mentions)
Smmc 7721, by American Type Culture Collection (23 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (22 mentions)
Bel 7402, by American Type Culture Collection (15 mentions)
Hep3b, by American Type Culture Collection (13 mentions)
Mhcc97h, by American Type Culture Collection (8 mentions)
Hcclm3, by American Type Culture Collection (8 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions)
Sk hep1, by American Type Culture Collection (6 mentions)
Qgy 7703, by American Type Culture Collection (5 mentions)
Hek293, by American Type Culture Collection (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (4 mentions)
Dmem medium, by Thermo Fisher Scientific (4 mentions)
Mcf 7, by American Type Culture Collection (3 mentions)
Hepg2, by Thermo Fisher Scientific (3 mentions)
Smmc 7721, by Thermo Fisher Scientific (3 mentions)
Hl 7702, by Thermo Fisher Scientific (3 mentions)
Bel 7404, by American Type Culture Collection (3 mentions)
Plc prf 5, by American Type Culture Collection (3 mentions)
74 protocols using hl 7702
1
Cell Culture Protocols for Cancer and Normal Liver Cells
2
Nanoparticle-based Therapeutic Delivery Protocol
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Dopamine hydrochloride and PTX were purchased from Sigma Aldrich Co., Ltd. Coumarin 6, IR780, and Pluronic F127 were purchased from Aladdin Co., Ltd. CCK-8 kit was purchased from Topscience Inc. DAPI kit was purchased from Biotech Ltd. The apoptosis detection kit was purchased from Beyotime Inc. Biotechnology. IL-6 detection kit and TNF-α test kit were purchased from Wuhan Servicebio Ltd. The other chemical reagents were purchased from Sinopharm Lnc. The Huh-7, BEL-7402, HepG-2, and HL-7702 Cell lines were all derived from ATCC. The BALB/C mice and BALB/c-nu/nu mice were purchased from Beijing HFK Biotechnology Ltd. The CB-17 SCID mouse was purchased from Charles River (Beijing) Experimental Animal Technology Ltd. Unless otherwise stated, all the chemicals and reagents were of analytical grade and used as received.
3
Cell Line Cultivation Protocol
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Human HCC cell lines (HepG2, Huh7, LM3 and Hep3B), the human immortalized liver cell line HL-7702, and the human embryonic kidney cell line HEK293T were purchased from ATCC (American type culture collection). These cells were maintained in their complete growth medium according to the culture method.
4
Hypoxia Exposure Protocol for HL-7702 Cells
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Human liver cells HL-7702 (ATCC) were cultured in DMEM/Ham’s F12 (v/v, 1:1) supplied with 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37 °C in a humidified atmosphere of 95% air and 5% CO2. The hypobaric hypoxia cell model was performed by incubating the culture under 1% O2, 94% N2, and 5% CO2 conditions. The cells were sealed and placed at 37 °C for 24 h, and after this harvested in RAPA lysis buffer and TRIZOL for downstream analyses.
5
Cell Culture Protocol for Leukemia Lines
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HEL (erythroleukemia), K562 (chronic myeloid leukemia), CEM-C7H2 (T-cell acute lymphoblastic leukemia), and HL7702 (normal hepatic cell line) were purchased from ATCC, Manassas, VA, United States . The cells were cultured in RPMI 1640 medium supplemented with 5% FBS at 37°C in a CO2 incubator (5% CO2 and 95% air, 95% humidity), as per standard conditions of passage.
6
Cell Culture Protocol for Huh7 and HL-7702
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7
Arsenic-Induced Oxidative Stress and Apoptosis
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Bulk arsenic was purchased from Sigma-Aldrich and stored in the dark with argon protection. Human normal liver cells (HL-7702), human embryonic kidney 293 (HEK293), human non-small cell lung cancer (A549), and human breast cancer cell (MCF-7) were obtained from ATCC. DPBF, MB, H2O2 (30%), glutathione, and N-methyl-pyrrolidone (NMP) were purchased from Sigma-Aldrich. RPMI 1640 medium, Dulbecco’s modified Eagle medium (DMEM), PBS, fetal bovine serum (FBS) and trypsin-EDTA were purchased from Gibco Life Technologies. Primary antibodies: Phospho-Histone H2A.X (Ser139) (D7T2V), Cell Signaling (Product # 80312), Dilution 1:200; C-CAS3 (Asp175) (5A1E), Cell Signaling (Product # 9664), Dilution 1:250. Secondary antibodies: Anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488, ThermoFisher (Catalog # A-11034), Dilution 1:1000; Anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647, ThermoFisher (Catalog # A-21236), Dilution 1:1000.
8
Quantitative Analysis and Bioactivity Evaluation of Citrus Flavonoids
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A Milli-Q water purification system (Millipore, Bedford, MA, USA); Agilent 1260 Series HPLC (Agilent Technologies, USA); Thermo BDS HYPERSIL C18 column (250 × 4.6 mm, 5 μm, Thermo, USA); Agilent 1100 Series HPLC (Agilent Technologies, USA); YMC-Pack ODS-A semi-preparative column (250 × 10 mm, 5 μm); nile red (Macklin, Shanghai, China); lovastatin (LOV; Aladdin, China); BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a, 4adiaza-s-indacene, Molecular Probes, Invitrogen); 5,7,8,4′-Tetramethoxyflavone (TET) (≥98%, Push bio-technology, China); narirutin (NAR), didymin (DID), nobiletin (NOB), sinensetin (SIN), 3,5,6,7,8,3′,4′-heptamethoxyflavone (HMF) and synephrine (SYN) (≥98%, Biopurify Phytochemicals, China); naringin (NAN) and hesperidin (HES) (≥98%, Must bio-technology, China); neohesperidin (NEO), 5-hydroxy-6,7,8,3′,4′-Pentamethoxyflavone and tangerine (TAN) (≥98%, prepared in our laboratory); TRIzol, high capacity cDNA reverse transcription kit and SYBR-green (Vazyme, Nanjing, China); quantitative PCR (Roche, Basel, Switzerland); HL7702 (ATCC, USA); Dulbecco’s modified Eagle’s medium (DMEM; Corning, USA). Fetal bovine serum (FBS; Gibco, USA); dimethyl sulfoxide (DMSO; Sigma, Louis, MO, USA.).
9
Cell Line Cultivation and Polydatin
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Normal liver cell lines HL-7702, HCC, and liver cancer cell lines HepG2 and SMMC-7721 were purchased from the ATCC company (USA). All the cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, USA). The cells were kept in the humidified incubator at 37°C with 5% CO2. Polydatin was purchased from Sigma (USA).
10
Characterization of Liver Cell Lines
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Human LC cells SMMC-7721 and HepG2 and normal liver cells HL-7702 were all bought from ATCC and preserved in CM1-1 medium. The three all grew adhering to the wall and were cultured in 5% CO2 at 37°C. SMMC-7721 was epithelial-like, polygonal, and short spindle-shaped. HepG2 was epithelial-like and clustered. HL-7702 was epithelial-like, polygonal, and round.
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