3h cort

Rat LH standard NIDDK-r-LH-I9 and LH antibody NIDDK-anti-rLH-S-11 were obtained through the National Hormone and Pituitary Program (Rockville, MD, USA). Radioactive ¹²⁵I-rat LH was obtained through Covance Laboratories (Vienna, VA, USA), and IgG antiserum was obtained from ICN Pharmaceuticals (Costa Mesa, CA, USA). ³H-CORT, ³H-pregnenolone, ³H-progesterone, ³H-androstenedione, ³H-CORT and ³H-testosterone were purchased from DuPont-New England Nuclear (Boston, MA, USA). The CORT antiserum B3-163 was obtained from Endocrine Sciences (Calabasas, CA, USA). NAD⁺ and NADPH were purchased from Sigma (St. Louis, MO, USA).

Rat LH standard NIDDK-r-LH-I9 was obtained through the National Hormone and Pituitary Program (United States). ³H-CORT, ³H-hydroxycholesterol, ³H-pregnenolone, ³H-progesterone, ³H-androstenedione, and ³H-T were purchased from DuPont-New England Nuclear (Boston, MA, United States). ³H-DHC was prepared as previously described (Lakshmi and Monder, 1985 (link)). The CORT antiserum B3-163 was obtained from Endocrine Sciences (Calabasas, CA, United States). NAD⁺ and NADPH were purchased from Sigma (St. Louis, MO, United States). RU486 was from Roussel (UCLAF, France). DHEA, 2-hydroxypropyl-cyclodextrin, etiocholan-3β-ol-17-one, M-199, DMEM, and Ham’s F-12 medium were purchased from Sigma (St. Louis, MO, United States).