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2 protocols using catechin

1

Analytical Characterization of Plant Extracts

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3-hexanone (chromatographic grade, Sigma-Aldrich); C8-C20 alkane mixture (Sigma-Aldrich); n-hexane (chromatographic grade, Ron); chloroform (AR, Sinopharm); Acetone (AR, Sinopharm); Folin-Ciocalteu (AR, Sinopharm); Gallic acid (AR, Sinopharm); Ascorbic acid (AR, Sinopharm); 2,2-diphenyl-1-picrylhydrazyl (AR, Sinopharm); 2,6-dichlorophenolindophenol (AR, Sinopharm); (+)-Catechin (AR, Sinopharm). 30 authentic volatile compound standards (Sigma-Aldrich).
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2

Comprehensive Antioxidant Assays Protocol

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RNA extraction kit was obtained from Beijing TransGen Co., Ltd., China, while qRT-PCR kit and reverse transcription kit were purchased from Beijing Takara Co., Ltd., China.
Chemical reagents (analytical grade), such as dibasic sodium phosphate (Na2HPO4), ferric chloride, potassium ferricyanide, ascorbic acid (AsA), hydrogen peroxide (H2O2), sodium diethyl dithiocarbamate trihydrate, sodium dihydrogen phosphate (NaH2PO4), polyvinyl pyrrolidone (PVP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxylamine hydrochloride, α-naphthyl amine, p-aminobenzenesulfonic acid, sodium nitrite, hydroxylamine, trichloroacetic acid, thibabituric acid, dithiothreitol, nitro blue tetrazolium, riboflavin, ethylenediaminetetraacetic acid disodium salt (EDTA-Na2), bovine serum albumin, catechin, gallic acid, methionine, dithio nitrobenzoic acid (DTNB), glutathione (GSH), methanol, were obtained from Shanghai Sinopharm Co. Ltd., China.
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