Vwf antibody

Paraffin-embedded sections of the right hemisphere were used for hematoxylin and eosin (HE) histological staining and immunohistochemisty, as described previously (10, 22) . Briefly, right cerebral hemisphere was fixed with 4% paraformaldehyde and coronally cut into 5-μm-thick sections, and then sections were stained with HE staining or used for IHC. For immunohistochemistry, paraffin sections were processed for heat-based antigen retrieval and endogenous peroxidase was inhibited with 0.3% hydrogen peroxide in methanol. Goat serum (1:10) served as the blocking agent before incubation with rabbit anti-HIF-1α antibody (1:50, Santa Cruz, Dallas, TX), rabbit anti-CC3 antibody (1:200, CST, Danvers, MA), rabbit anti-VEGF antibody (1:200, Santa Cruz), and rabbit anti-von willebrand factor (vwf) antibody (1:200, Millipore, Darmstadt, Germany). After incubation overnight at 4°C, biotin-labeled secondary antibody work solution (ZSGB-BIO, Beijing, China) was loaded, followed by freshly prepared ABC (avidin biotin complex, ZSGB-BIO), and then 3-3' diaminobenzidine (DAB, KPL, Gaithersburg, MD) was used as a chromogen. Finally, sections were counter-stained with hematoxylin.